ArticleMolecular and cellular biology1993
Inactive chromatin spreads from a focus of methylation.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 122 citations in OpenAlex.
- Silencing of Unintegrated Retroviral DNAs.Viruses · 2021Review
- CpG Dinucleotides Inhibit HIV-1 Replication through Zinc Finger Antiviral Protein (ZAP)-Dependent and -Independent Mechanisms.Journal of virology · 2020Article
- Investigation of MORC1 DNA methylation as biomarker of early life stress and depressive symptoms.Journal of psychiatric research · 2020Article
- Histone deacetylase inhibitors reactivate silenced transgene in vivo.Gene therapy · 2019Article
- Increasing the CpG dinucleotide abundance in the HIV-1 genomic RNA inhibits viral replication.Retrovirology · 2017Article
- Development of S/MAR minicircles for enhanced and persistent transgene expression in the mouse liver.Journal of molecular medicine (Berlin, Germany) · 2011Article
- The effects of EBV transformation on gene expression levels and methylation profiles.Human molecular genetics · 2011Article
- Infrequent methylation of the DUSP6 phosphatase in endometrial cancer.Gynecologic oncology · 2010Article
- Methylation-dependent binding of the epstein-barr virus BZLF1 protein to viral promoters.PLoS pathogens · 2009Article
- Frequent but borderline methylation of p16 (INK4a) and TIMP3 in medulloblastoma and sPNET revealed by quantitative analyses.Journal of neuro-oncology · 2007Article
- DNA methylation dictates histone H3K4 methylation.Molecular and cellular biology · 2007Article
- An unmethylated 3' promoter-proximal region is required for efficient transcription initiation.PLoS genetics · 2007Article
- Metallothionien 3 expression is frequently down-regulated in oesophageal squamous cell carcinoma by DNA methylation.Molecular cancer · 2005Article
- BZLF1 activation of the methylated form of the BRLF1 immediate-early promoter is regulated by BZLF1 residue 186.Journal of virology · 2005Article
- Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus.Nucleic acids research · 2005Article
- DNA methylation has a local effect on transcription and histone acetylation.Molecular and cellular biology · 2002Article
- Tissue-specific silencing of a transgene in rice.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Molecular mechanisms of gene silencing mediated by DNA methylation.Molecular and cellular biology · 2002Article
- Methylation-mediated proviral silencing is associated with MeCP2 recruitment and localized histone H3 deacetylation.Molecular and cellular biology · 2001Article
- Reversal of methylation-mediated repression with short-chain fatty acids: evidence for an additional mechanism to histone deacetylation.Nucleic acids research · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The detailed mechanisms of inhibition of transcription by DNA methylation are still unknown, but it has become obvious that the formation of chromatin plays an important role in this process. Using an approach enabling us to methylate, in vitro, chosen regions in a plasmid, we now show that specific methylation of nonpromoter sequences results in transcriptional inhibition of a reporter gene construct and that this inhibition is independent of the position of the methylated region within the plasmid. In plasmid minichromosomes containing a short region of methylated DNA, both methylated and unmethylated sequences are protected from limited MspI digestion. Our results show that inactive chromatin is present at unmethylated regions in partially methylated minichromosomes and can thereby inhibit gene expression. Spreading of the inactive chromatin is not inhibited by the presence of active promoters, nor is it a consequence of transcriptional inactivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.