ArticleAnnals of biomedical engineering
Effect of cytochalasin D on the mechanical properties and morphology of passive human neutrophils.
Article in Annals of biomedical engineering. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 64 citations in OpenAlex.
- Candida albicans aspartyl protease (Sap6) inhibits neutrophil function via a "Trojan horse" mechanism.Scientific reports · 2025Article
- Cellular Uptake of Phase-Separating Peptide Coacervates.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- A Perspective on Developing Modeling and Image Analysis Tools to Investigate Mechanosensing Proteins.Integrative and comparative biology · 2023Article
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- Structural Configuration of Blood Cell Membranes Determines Their Nonlinear Deformation Properties.BioMed research international · 2022Article
- Impaired macrophage trafficking and increased helper T-cell recruitment with loss of cadherin-11 in atherosclerotic immune response.American journal of physiology. Heart and circulatory physiology · 2021Article
- Comparison of Serotonin-Regulated Calcific Processes in Aortic and Mitral Valvular Interstitial Cells.ACS omega · 2021Article
- Activation of NLRP3 by uropathogenic Escherichia coli is associated with IL-1β release and regulation of antimicrobial properties in human neutrophils.Scientific reports · 2020Article
- Qualitative analysis of contribution of intracellular skeletal changes to cellular elasticity.Cellular and molecular life sciences : CMLS · 2020Review
- Correlative Fluorescence- and Electron Microscopy of Whole Breast Cancer Cells Reveals Different Distribution of ErbB2 Dependent on Underlying Actin.Frontiers in cell and developmental biology · 2020Article
- Identification of novel macropinocytosis inhibitors using a rational screen of Food and Drug Administration-approved drugs.British journal of pharmacology · 2018Article
- Quantitative Deformability Cytometry: Rapid, Calibrated Measurements of Cell Mechanical Properties.Biophysical journal · 2017Article
- High-throughput linear optical stretcher for mechanical characterization of blood cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2016Article
- Article
- Controlled One-on-One Encounters between Immune Cells and Microbes Reveal Mechanisms of Phagocytosis.Biophysical journal · 2015Review
- Nuclear envelope composition determines the ability of neutrophil-type cells to passage through micron-scale constrictions.The Journal of biological chemistry · 2013Article
- A dual role for diacylglycerol kinase generated phosphatidic acid in autoantibody-induced neutrophil exocytosis.Molecular medicine (Cambridge, Mass.) · 2011Article
- A semianalytical model to study the effect of cortical tension on cell rolling.Biophysical journal · 2010Article
- Animal cell hydraulics.Journal of cell science · 2009Article
- Human neutrophil surface protrusion under a point load: location independence and viscoelasticity.American journal of physiology. Cell physiology · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The actin-rich cortex plays a major role in neutrophil chemotaxis and phagocytosis. In passive neutrophils, 30-50% of the actin molecules are in the F (filamentous) form, and it is the shifting of equilibrium with its monomeric G (globular) form that controls cell motility and phagocytosis. Cytochalasins have been shown to inhibit cell phagocytosis and ruffling. In purified actin, cytochalasins have been shown to decrease the amount of F-actin by capping the fast-growth end of actin filaments. Recent studies with intact cells, however, reveal that the most potent cytochalasin, cytochalasin D (CD), actually increases F-actin content suggesting that CD disrupts the actin network so as to increase the number of actin-filament ends for further actin polymerization. In this paper, we report the effects of CD on the passive mechanical behavior and morphology of human neutrophils with 1, 2, 10, and 20 microM CD. At 1 and 2 microM CD, the cells remain spherical. However, in the presence of 10 and 20 microM CD, cells are severely deformed and "blebby" as shown by light and scanning electron microscopy. After 1 and 2 microM CD treatment, the cells show a decrease of 43 and 66%, respectively, in cortical tension when measured by static micropipet aspiration experiments. Similarly, the cytoplasmic viscosities of 1 and 2 microM CD-treated cells are decreased, but only by 17 and 24%, respectively. A proportionally greater effect on the cortical tension suggests that CD acts mainly on the actin-rich cortex by disrupting the filament network.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.