ArticleGenetics1995
A novel positive selection for identifying cold-sensitive myosin II mutants in Dictyostelium.
Article in Genetics, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- WASP family proteins and formins compete in pseudopod- and bleb-based migration.The Journal of cell biology · 2018Article
- Selective localization of myosin-I proteins in macropinosomes and actin waves.Cytoskeleton (Hoboken, N.J.) · 2016Article
- Molecular basis of dynamic relocalization of Dictyostelium myosin IB.The Journal of biological chemistry · 2012Article
- Structural basis for the allosteric interference of myosin function by reactive thiol region mutations G680A and G680V.The Journal of biological chemistry · 2011Article
- A new environmentally resistant cell type from Dictyostelium.Microbiology (Reading, England) · 2007Article
- The early history of the biochemistry of muscle contraction.The Journal of general physiology · 2004Article
- A streamlined process to phenotypically profile heterologous cDNAs in parallel using yeast cell-based assays.Genome research · 2001Article
- Actin dynamics at the living cell submembrane imaged by total internal reflection fluorescence photobleaching.Biophysical journal · 2000Article
- Dynacortin, a genetic link between equatorial contractility and global shape control discovered by library complementation of a Dictyostelium discoideum cytokinesis mutant.The Journal of cell biology · 2000Article
- A structural model for phosphorylation control of Dictyostelium myosin II thick filament assembly.The Journal of cell biology · 1999Article
- Myosin I contributes to the generation of resting cortical tension.Biophysical journal · 1999Article
- Article
- The myosin I SH3 domain and TEDS rule phosphorylation site are required for in vivo function.Molecular biology of the cell · 1998Article
- Dictyostelium RasG is required for normal motility and cytokinesis, but not growth.The Journal of cell biology · 1997Article
- The structure of the acto-myosin subfragment 1 complex: results of searches using data from electron microscopy and x-ray crystallography.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Myosin I overexpression impairs cell migration.The Journal of cell biology · 1997Article
- Myosin light chain kinase (MLCK) gene disruption in Dictyostelium: a role for MLCK-A in cytokinesis and evidence for multiple MLCKs.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Cold-sensitive mutations of Dictyostelium myosin heavy chain highlight functional domains of the myosin motor.Genetics · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We developed a positive selection for myosin heavy chain mutants in Dictyostelium. This selection is based on the fact that brief exposure to azide causes wild-type cells to release from the substrate, whereas myosin null cells remain adherent. This procedure assays myosin function on a time scale of minutes and has therefore allowed us to select rapid-onset cold-sensitive mutants after random chemical mutagenesis of Dictyostelium cells. We developed a rapid technique for determining which mutations lie in sequences of the myosin gene that encode the head (motor) domain and localized 27 of 34 mutants to this domain. We recovered the appropriate sequences from five of the mutants and demonstrated that they retain their cold-sensitive properties when expressed from extrachromosomal plasmids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.