ArticlePharmaceutical research1995
Permeability of peptides and proteins in human cultured alveolar A549 cell monolayer.
Article in Pharmaceutical research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- DS-3939a: A TA-MUC1-Directed Antibody-Drug Conjugate with Broad Antitumor Activity.Molecular cancer therapeutics · 2026Article
- In vitro tools for orally inhaled drug products-state of the art for their application in pharmaceutical research and industry and regulatory challenges.In vitro models · 2022Review
- Permeability Properties of anCellular and molecular bioengineering · 2021Article
- Permeability of Epithelial/Endothelial Barriers in Transwells and Microfluidic Bilayer Devices.Micromachines · 2019Article
- Barrier-protective effects of activated protein C in human alveolar epithelial cells.PloS one · 2013Article
- Assessment of cell line models of primary human cells by Raman spectral phenotyping.Biophysical journal · 2010Article
- Epidermal growth factor receptor and claudin-2 participate in A549 permeability and remodeling: implications for non-small cell lung cancer tumor colonization.Molecular carcinogenesis · 2009Article
- Continuous monitoring of the bronchial epithelial lining fluid by microdialysis.Respiratory research · 2007Article
- Interplay of secondary structure and charge on the diffusion of a polypeptide through negatively charged aqueous pores.Pharmaceutical research · 2007Article
- A randomly coiled, high-molecular-weight polypeptide exhibits increased paracellular diffusion in vitro and in situ relative to the highly ordered alpha-helix conformer.Pharmaceutical research · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe transport of peptides or proteins across the alveolar cell monolayer was studied in vitro in order to elucidate their transport pathway.
methodsThe permeability of 14 peptides or proteins and 6 dextrans with MW 1,000-150,000 was measured in cultured human lung adenocarcinoma A549 cell monolayers at 37 degrees C or 4 degrees C. The stability of the tested peptides and proteins was also evaluated.
resultsThe permeability coefficients of these macromolecules across the A549 cell monolayer at 37 degrees C ranged from 10(-5) to 10(-7) (cm/sec), and exhibited a good inverse correlation with molecular weight. All macromolecules were stable throughout the transport experiment, and degradation by proteases was minimal. Permeability at 4 degrees C did not differ from that at 37 degrees C. Clear selectivity for direction of transport was not observed.
conclusionsThese results suggested that the tested peptides and proteins appeared to penetrate the A549 cell monolayer via a paracellular route by passive diffusion.
Indexed as
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7494821What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.