ArticleJournal of virology1995
Novel retroviral vectors for efficient expression of the multidrug resistance (mdr-1) gene in early hematopoietic cells.
Article in Journal of virology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 233 citations in OpenAlex.
- Oncogenesis as an Adverse Effect of Gene Replacement Therapy in Hematopoietic Stem Cells.International journal of molecular sciences · 2026Review
- Long Terminal Repeats of Gammaretroviruses Retain Stable Expression after Integration Retargeting.Viruses · 2024Article
- Promoter considerations in the design of lentiviral vectors for use in treating lysosomal storage diseases.Molecular therapy. Methods & clinical development · 2022Review
- Retroviral gene therapy in Germany with a view on previous experience and future perspectives.Gene therapy · 2021Review
- HLA Class I Knockout Converts Allogeneic Primary NK Cells Into Suitable Effectors for "Off-the-Shelf" Immunotherapy.Frontiers in immunology · 2020Article
- Hepatocyte-like cells generated by direct reprogramming from murine somatic cells can repopulate decellularized livers.Biotechnology and bioengineering · 2018Article
- Article
- Heterologous viral promoters incorporated into the human cytomegalovirus genome are silenced during experimental latency.Journal of virology · 2013Article
- Selection for Evi1 activation in myelomonocytic leukemia induced by hyperactive signaling through wild-type NRas.Oncogene · 2013Article
- Influenza virus-specific TCR-transduced T cells as a model for adoptive immunotherapy.Human vaccines & immunotherapeutics · 2013Article
- Displaying high-affinity ligands on adeno-associated viral vectors enables tumor cell-specific and safe gene transfer.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Lentiviral vector induced insertional haploinsufficiency of Ebf1 causes murine leukemia.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- Pantropic retroviruses as a transduction tool for sea urchin embryos.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A retroviral vector suitable for ultrasound image-guided gene delivery to mouse brain.Gene therapy · 2012Article
- Scale-up and manufacturing of clinical-grade self-inactivating γ-retroviral vectors by transient transfection.Gene therapy · 2012Article
- Gene therapy: light is finally in the tunnel.Protein & cell · 2011Review
- Correction of murine SCID-X1 by lentiviral gene therapy using a codon-optimized IL2RG gene and minimal pretransplant conditioning.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- Review
- Lentiviral vector design and imaging approaches to visualize the early stages of cellular reprogramming.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present data that retroviral gene expression in early hematopoietic cells is subjected to transcriptional controls similar to those previously described for embryonic stem cells. Transient transfection experiments revealed that both the viral enhancer region in the U3 region of the long terminal repeat as well as a repressor element coincident with the primer binding site of Moloney leukemia viruses are limiting for expression in hematopoietic cells in a differentiation-dependent manner. Within the group of Moloney leukemia virus-related viruses, only the myeloproliferative sarcoma virus showed high enhancer activity in myeloid (including erythroid) cells. In contrast, enhancer regions related to the Friend mink cell focus-forming viruses mediate much higher gene expression levels in both multipotent and lineage-committed myeloid cells. In addition, transcriptional repression related to sequences in the primer binding site of Moloney leukemia virus-derived vectors is also found in early hematopoietic cells and can be overcome by using the corresponding sequences of the murine embryonic stem cell virus. On the basis of these results, two types of novel retroviral hybrid vectors were developed; they combine the U3 regions of either the Friend mink cell focus-forming virus family or the myeloproliferative sarcoma virus with the primer binding site of the murine embryonic stem cell virus. When used to express the human multiple drug resistance gene, these vectors substantially improve protection to cytostatic drugs in transduced hematopoietic cell lines FDC-Pmix, TF-1, and K-562 in comparison with Moloney leukemia virus-derived vectors presently used for the stem cell protection approach in somatic gene therapy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.