ArticleAnnals of the rheumatic diseases1995
Human blood and synovial fluid neutrophils cultured in vitro undergo programmed cell death which is promoted by the addition of synovial fluid.
Article in Annals of the rheumatic diseases, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 30 citations in OpenAlex.
- Hyaluronic Acid in Synovial Fluid Prevents Neutrophil Activation in Spondyloarthritis.International journal of molecular sciences · 2023Article
- Model-Based Discovery and Development of Biopharmaceuticals: A Case Study of Mavrilimumab.CPT: pharmacometrics & systems pharmacology · 2018Article
- Inflammatory response of cultured rat synoviocytes challenged with synovial fluid from osteoarthritis patients correlates with their radiographic grading: a pilot study.In vitro cellular & developmental biology. Animal · 2015Article
- Galectins in the Pathogenesis of Rheumatoid Arthritis.Journal of clinical & cellular immunology · 2013Article
- Regulation of neutrophil apoptosis differs after in vivo transmigration to skin chambers and synovial fluid: a role for inflammasome-dependent interleukin-1β release.Journal of innate immunity · 2013Article
- Pivotal Advance: Pharmacological manipulation of inflammation resolution during spontaneously resolving tissue neutrophilia in the zebrafish.Journal of leukocyte biology · 2010Article
- Leflunomide inhibits transendothelial migration of peripheral blood mononuclear cells.Annals of the rheumatic diseases · 2004Article
- Increased apoptotic peripheral blood neutrophils in systemic lupus erythematosus: relations with disease activity, antibodies to double stranded DNA, and neutropenia.Annals of the rheumatic diseases · 1999Article
- Modulation by proinflammatory cytokines of Fas/Fas ligand-mediated apoptotic cell death of synovial cells in patients with rheumatoid arthritis (RA).Clinical and experimental immunology · 1998Article
- Arachidonic acid induces DNA-fragmentation in human polymorphonuclear neutrophil granulocytes.Inflammation · 1997Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo assess the influence of inflammatory synovial fluid (SF) on apoptosis of joint and blood neutrophils with particular reference to levels of colony stimulating factors (CSF) contained therein.
methodsNeutrophils were separated from fresh synovial fluid and from peripheral blood by density gradient centrifugation. Apoptosis was assayed by light microscope morphology and DNA degradation. CSFs were assayed using bone marrow bioassay and enzyme linked immunosorbent assays for granulocyte (G-) and granulocyte macrophage (GM-) CSF. Separated neutrophils were cultured in vitro and exposed to: varying concentrations of SF in which CSF levels were measured, recombinant G-CSF and GM-CSF, and hyaluronic acid control solutions. Numbers of apoptotic neutrophils and CSF levels were also measured in fresh SF samples.
resultsThe addition of autologous or heterologous inflammatory SF to blood or joint cavity neutrophils cultured in vitro caused a significant dose dependent increase in the percentage of cells becoming apoptotic with time as measured morphologically and confirmed by DNA degradation. The effect bore no relationship to levels of CSF in joint fluid, despite our finding that GM-CSF produced inhibition of neutrophil apoptosis in vitro.
conclusionThese data suggest that SF contains a factor or factors capable of directly or indirectly promoting neutrophil apoptosis and normally powerful enough to overcome the apoptosis inhibiting effects of cytokines such as GM-CSF at concentrations usually found in inflammatory synovial fluids.
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