ArticleAnnals of the rheumatic diseases1995
Enhanced membrane expression of the 52 kDa Ro(SS-A) and La(SS-B) antigens by human keratinocytes induced by TNF alpha.
Article in Annals of the rheumatic diseases, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 71 citations in OpenAlex.
- Cutaneous lupus erythematosus - from pathogenesis to targeted therapy.Nature reviews. Rheumatology · 2025Review
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- Route of Application and Dose Evaluation of Dental Pulp Stem Cells for the Treatment of Sialadenitis Caused by Sjögren's Syndrome: A Preclinical Study.Biomedicines · 2025Article
- Current concepts of photosensitivity in cutaneous lupus erythematosus.Frontiers in medicine · 2022Review
- Immunobiology of T Cells in Sjögren's Syndrome.Clinical reviews in allergy & immunology · 2021Review
- Molecular Mechanism of Sphingosine-1-Phosphate Receptor 1 Regulating CD4International journal of general medicine · 2021Article
- Inhibition of Cathepsin S Reduces Lacrimal Gland Inflammation and Increases Tear Flow in a Mouse Model of Sjögren's Syndrome.Scientific reports · 2019Article
- Article
- Pathogenic role of endogenous TNF-α in the development of Sjögren's-like sialadenitis and secretory dysfunction in non-obese diabetic mice.Laboratory investigation; a journal of technical methods and pathology · 2017Article
- Passive transfer of antibodies to the linear epitope 60 kD Ro 273-289 induces features of Sjögren's syndrome in naive mice.Clinical and experimental immunology · 2015Article
- T Cell-Associated Cytokines in the Pathogenesis of Sjögren's Syndrome.Journal of clinical & cellular immunology · 2013Article
- Clinical and pathological roles of Ro/SSA autoantibody system.Clinical & developmental immunology · 2012Review
- Autoantigen TRIM21/Ro52 as a Possible Target for Treatment of Systemic Lupus Erythematosus.International journal of rheumatology · 2012Article
- Vasculitis: mechanisms involved and clinical manifestations.Arthritis research & therapy · 2007Review
- Anti-SSB/La is one of the antineutrophil autoantibodies responsible for neutropenia and functional impairment of polymorphonuclear neutrophils in patients with systemic lupus erythematosus.Clinical and experimental immunology · 2003Article
- Exposure of HEp-2 cells to stress conditions influences antinuclear antibody reactivity.Clinical and diagnostic laboratory immunology · 2002Article
- SS-56, a novel cellular target of autoantibody responses in Sjögren syndrome and systemic lupus erythematosus.The Journal of clinical investigation · 2001Article
- Tumour necrosis factor and other cytokines in murine lupus.Annals of the rheumatic diseases · 1999Review
- La autoantigen specifically recognizes a predicted stem-loop in hepatitis B virus RNA.Journal of virology · 1999Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo investigate the membrane expression of the 52 kDa Ro(SS-A) and La(SS-B) antigens in human keratinocytes under the influence of an important mediator of inflammation, TNF alpha.
methodsKeratinocytes, isolated from human skins obtained at circumcision and identified using monoclonal antibodies, were treated with tumour necrosis factor alpha (TNF alpha) and incubated with antibodies to 52 kDa Ro(SS-A) isolated and purified from patients with systemic lupus erythematosus or Sjögren's syndrome, with mouse monoclonal antibody to La(SS-B), and (as controls) with sera from normal healthy blood donors and a mouse monoclonal antibody to U1RNP 68 kDa. Membrane expression of the 52 kDa Ro(SS-A) and La(SS-B) antigens was detected using cyto enzyme linked immunosorbent assays (ELISAs), laser scanning microscopy, and indirect immunofluorescence.
resultsAfter the incubation with TNF alpha, cyto ELISA revealed a significantly increased membrane binding of 52 kDa Ro(SS-A) antibodies, with a maximum after two hours, followed by enhanced 52 kDa Ro(SS-A) expression during the subsequent 24 hours. The La(SS-B) antigen was expressed rapidly after TNF alpha treatment (within one hour), with a fast decrease to the preincubation value within three hours. Indirect immunofluorescence with fixed normal human keratinocytes confirmed increased 52 kDa Ro(SS-A) and La(SS-B) antigen expression after the incubation with TNF alpha.
conclusionsTNF alpha mediates 52 kDa Ro(SS-A) and La(SS-B) autoantigen surface expression on human keratinocytes, and may be an important factor both in antibody induction and in the initiation of immunopathogenic processes which occur after antibody binding in autoimmune dermatitis.
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