ArticlePflugers Archiv : European journal of physiology1995
Effects of polyamines on voltage-activated calcium channels in guinea-pig intestinal smooth muscle.
Article in Pflugers Archiv : European journal of physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- The Potential Role of Polyamines in Epilepsy and Epilepsy-Related Pathophysiological Changes.Biomolecules · 2022Review
- Polyamine block of MthK potassium channels.The Journal of general physiology · 2020Article
- Analysis of interaction between intracellular spermine and transient receptor potential canonical 4 channel: multiple candidate sites of negatively charged amino acids for the inward rectification of transient receptor potential canonical 4.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2020Article
- Polyamine-mediated channel block of ionotropic glutamate receptors and its regulation by auxiliary proteins.The Journal of biological chemistry · 2018Review
- Stargazin and cornichon-3 relieve polyamine block of AMPA receptors by enhancing blocker permeation.The Journal of general physiology · 2018Article
- Polyamines as Snake Toxins and Their Probable Pharmacological Functions in Envenomation.Toxins · 2016Article
- Kainate receptor pore-forming and auxiliary subunits regulate channel block by a novel mechanism.The Journal of physiology · 2016Article
- Polyamines and their metabolites as diagnostic markers of human diseases.Biomolecules & therapeutics · 2013Review
- Diabetes-induced inhibition of voltage-dependent calcium channels in the retinal microvasculature: role of spermine.Investigative ophthalmology & visual science · 2010Article
- Ca(2+) channels on the move.Biochemistry · 2009Review
- Relaxant Effect of Spermidine on Acethylcholine and High K-induced Gastric Contractions of Guinea-Pig.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2008Article
- Effects of polyamines on contractility of guinea-pig gastric smooth muscle.Journal of Korean medical science · 2007Article
- Effects of polyamines on the muscarinic receptor-operated cation current in guinea-pig ileal smooth muscle myocytes.British journal of pharmacology · 2004Article
- Monovalent cation (MC) current in cardiac and smooth muscle cells: regulation by intracellular Mg2+ and inhibition by polycations.British journal of pharmacology · 2003Article
- The EEEE locus is the sole high-affinity Ca(2+) binding structure in the pore of a voltage-gated Ca(2+) channel: block by ca(2+) entering from the intracellular pore entrance.The Journal of general physiology · 2000Article
- External action of di- and polyamines on maxi calcium-activated potassium channels: an electrophysiological and molecular modeling study.Biophysical journal · 1998Article
- Putrescine-stimulated intracellular Ca2+ release for invasiveness of rat ascites hepatoma cells.Japanese journal of cancer research : Gann · 1998Article
- Sensitivity of cloned muscle, heart and neuronal voltage-gated sodium channels to block by polyamines: a possible basis for modulation of excitability in vivo.Channels (Austin, Tex.)Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Effects of polyamines on the spontaneous mechanical and electrical activity of guinea-pig intestinal smooth muscle were studied. Spermine and spermidine inhibited action potential generation and contractions, while putrescine had no effect. Single smooth muscle cells were isolated from the longitudinal muscle layer of the guinea-pig ileum. Whole-cell voltage-clamp experiments were carried out to investigate the effects of polyamines on current through voltage-activated Ca2+ channels. Spermine and spermidine (0.1-1 mM) reduced the inward current in a concentration-dependent manner. Spermine blocked current activated by the dihydropyridine agonist BAY K 8644 (1 microM), whereas no additional inhibition by spermine was seen after blockage of dihydropyridine-sensitive channels by nifedipine (0.1 microM). Inhibition by spermine or spermidine did not shift the peak of the current voltage relation of the inward current. Steady-state activation and inactivation relationships were not affected and thus the amplitude, but not the voltage dependence, of the window current responsible for Ca2+ inflow during sustained depolarization was affected. Putrescine (1 mM) had no significant effect on the inward current. These results suggest that spermine and spermidine inhibit contraction in spontaneously active intestinal smooth muscle by inhibiting Ca2+ current responsible for generation of action potentials.
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