ArticlePflugers Archiv : European journal of physiology1995
On the regulation of the expressed L-type calcium channel by cAMP-dependent phosphorylation.
Article in Pflugers Archiv : European journal of physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 89 citations in OpenAlex.
- Endogenous ion channels expressed in human embryonic kidney (HEK-293) cells.Pflugers Archiv : European journal of physiology · 2022Review
- The quest to identify the mechanism underlying adrenergic regulation of cardiac CaChannels (Austin, Tex.) · 2020Review
- β-adrenergic-mediated dynamic augmentation of sarcolemmal CaThe Journal of physiology · 2019Article
- Protein kinase A regulates C-terminally truncated CaThe Journal of physiology · 2017Article
- Regulation of Cardiac Calcium Channels in the Fight-or-Flight Response.Current molecular pharmacology · 2015Review
- Manipulating L-type calcium channels in cardiomyocytes using split-intein protein transsplicing.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- CaV1.2 signaling complexes in the heart.Journal of molecular and cellular cardiology · 2013Review
- Tetraspanin-13 modulates voltage-gated CaV2.2 Ca2+ channels.Scientific reports · 2013Article
- Molecular mechanism of calcium channel regulation in the fight-or-flight response.Science signaling · 2010Article
- Molecular mechanisms, and selective pharmacological rescue, of Rem-inhibited CaV1.2 channels in heart.Circulation research · 2010Article
- Supramolecular assemblies and localized regulation of voltage-gated ion channels.Physiological reviews · 2009Review
- Unchanged beta-adrenergic stimulation of cardiac L-type calcium channels in Ca v 1.2 phosphorylation site S1928A mutant mice.The Journal of biological chemistry · 2008Article
- Beta-adrenergic stimulation of L-type Ca2+ channels in cardiac myocytes requires the distal carboxyl terminus of alpha1C but not serine 1928.Circulation research · 2006Article
- Direct and remote modulation of L-channels in chromaffin cells: distinct actions on alpha1C and alpha1D subunits?Molecular neurobiology · 2004Review
- Regulation of L-type Ca2+ channels in the heart: overview of recent advances.Molecular and cellular biochemistry · 2003Review
- Beta-adrenergic regulation requires direct anchoring of PKA to cardiac CaV1.2 channels via a leucine zipper interaction with A kinase-anchoring protein 15.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Calcineurin enhances L-type Ca(2+) channel activity in hippocampal neurons: increased effect with age in culture.Neuroscience · 2002Article
- Modulation of the smooth-muscle L-type Ca2+ channel alpha1 subunit (alpha1C-b) by the beta2a subunit: a peptide which inhibits binding of beta to the I-II linker of alpha1 induces functional uncoupling.The Biochemical journal · 2000Article
- cAMP-dependent phosphorylation sites and macroscopic activity of recombinant cardiac L-type calcium channels.Molecular and cellular biochemistry · 1998Article
- Post-translational modifications of beta subunits of voltage-dependent calcium channels.Journal of bioenergetics and biomembranes · 1998Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Ca2+ channel subunits alpha 1C-a and alpha 1C-b were stably expressed in Chinese hamster ovary (CHO) and human embryonic kidney (HEK) 293 cells. The peak Ba2+ current (IBa) of these cells was not affected significantly by internal dialysis with 0.1 mM cAMP-dependent protein kinase inhibitor peptide (mPKI), 25 microM cAMP-dependent protein kinase catalytic subunit (PKA), or a combination of 25 microM PKA and 1 microM okadaic acid. The activity of the alpha 1C-b channel subunit expressed stably in HEK 293 cells was depressed by 1 microM H 89 and was not increased by superfusion with 5 microM forskolin plus 20 microM isobutyl-methylxanthine (IBMX). The alpha 1C-a.beta 2.alpha 2/delta complex was transiently expressed in HEK 293 cells; it was inhibited by internal dialysis of the cells with 1 microM H 89, but was not affected by internal dialysis with mPKI, PKA or microcystin. Internal dialysis of cells expressing the alpha 1C-a.beta 2.alpha 2/delta channel with 10 microM PKA did not induce facilitation after a 150-ms prepulse to +50 mV. The Ca2+ current (ICa) of cardiac myocytes increased threefold during internal dialysis with 5 microM PKA or 25 microM microcystin and during external superfusion with 0.1 microM isoproterenol or 5 microM forskolin plus 50 microM IBMX. These results indicate that the L-type Ca2+ channel expressed is not modulated by cAMP-dependent phosphorylation to the same extent as in native cardiac myocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.