ArticlePflugers Archiv : European journal of physiology1995
Different modulation of Ca-activated K channels by the intracellular redox potential in pulmonary and ear arterial smooth muscle cells of the rabbit.
Article in Pflugers Archiv : European journal of physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 71 citations in OpenAlex.
- Potassium channels in vascular smooth muscle cells.Physiological reviews · 2026Review
- Redox Regulation of Ion Channels and Receptors in Pulmonary Hypertension.Antioxidants & redox signaling · 2019Review
- Hypoxia-dependent reactive oxygen species signaling in the pulmonary circulation: focus on ion channels.Antioxidants & redox signaling · 2015Review
- Redox regulation of ion channels in the pulmonary circulation.Antioxidants & redox signaling · 2015Review
- Role of in vivo vascular redox in resistance arteries.Hypertension (Dallas, Tex. : 1979) · 2015Article
- Distinct activity of BK channel β1-subunit in cerebral and pulmonary artery smooth muscle cells.American journal of physiology. Cell physiology · 2013Article
- Hypoxic pulmonary vasoconstriction.Physiological reviews · 2012Review
- Role of ROS signaling in differential hypoxic Ca2+ and contractile responses in pulmonary and systemic vascular smooth muscle cells.Respiratory physiology & neurobiology · 2010Article
- Mitochondria, oxidative stress, and temporal lobe epilepsy.Epilepsy research · 2010Review
- Oxygen-induced tension in the sheep ductus arteriosus: effects of gestation on potassium and calcium channel regulation.Pediatric research · 2009Article
- Oxidant and redox signaling in vascular oxygen sensing: implications for systemic and pulmonary hypertension.Antioxidants & redox signaling · 2008Review
- Mitochondrial reactive oxygen species inactivate neuronal nicotinic acetylcholine receptors and induce long-term depression of fast nicotinic synaptic transmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Hypoxia reduces KCa channel activity by inducing Ca2+ spark uncoupling in cerebral artery smooth muscle cells.American journal of physiology. Cell physiology · 2007Article
- A cysteine-rich motif confers hypoxia sensitivity to mammalian large conductance voltage- and Ca-activated K (BK) channel alpha-subunits.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Mitochondria-derived reactive oxygen species dilate cerebral arteries by activating Ca2+ sparks.Circulation research · 2005Article
- Acute hypoxia differentially regulates K(+) channels. Implications with respect to cardiac arrhythmia.European biophysics journal : EBJ · 2005Review
- Sodium (2-sulfonatoethyl) methanethiosulfonate prevents S-nitroso-L-cysteine activation of Ca2+-activated K+ (BKCa) channels in myocytes of the guinea-pig taenia caeca.British journal of pharmacology · 2003Article
- Oxidative regulation of large conductance calcium-activated potassium channels.The Journal of general physiology · 2001Article
- Oxygen-sensing persistent sodium channels in rat hippocampus.The Journal of physiology · 2000Article
- Nitric oxide and thiol reagent modulation of Ca2+-activated K+ (BKCa) channels in myocytes of the guinea-pig taenia caeci.The Journal of physiology · 2000Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the electrical responses of Ca-activated K (KCa) currents induced by hypoxia and reduction or oxidation of the channel protein in pulmonary (PASMC) and ear (EASMC) arterial smooth muscle cells using the patch-clamp technique. In cell-attached patches, in the presence of a high K solution (containing 0.316 microM Ca2+), the activity of KCa channels from PASMC was decreased (by 49 +/- 7% compared to control, pipette potential = -70 mV) by changing to a hypoxic solution (1 mM Na2S2O4, aeration with 100% N2 gas). EASMC channels did not respond to hypoxia. In order to investigate the possible mechanisms involved, using inside-out patches bathed symmetrically in 150 mM KCl, we applied redox couples to the intracellular side. Reducing agents, such as dithiothreitol (DDT, 5 mM), reduced glutathione, (GSH, 5 mM), and nicotinamide adenine dinucleotide reduced (NADH, 2 mM) decreased PASMC, but not EASMC, KCa channel activity. However, oxidizing agents such as 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB, 1 mM), oxidized glutathione (GSSG, 5 mM) and NAD (2 mM) increased KCa channel activity in both PASMC and EASMC. The increased activity due to oxidizing agents was restored by applying reducing agents. From these results, we could suggest that the basal redox state of the EASMC KCa channel is more reduced than that of the PASMC channel, since the response of KCa channels of the EASMC to intracellular reducing agents differs from that of the PASMC. This difference may be related to the different responses of PASMC and EASMC KCa channels to hypoxia.
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