ArticleThe Journal of cell biology1995
Binding of E-MAP-115 to microtubules is regulated by cell cycle-dependent phosphorylation.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 60 citations in OpenAlex.
- Structure and mechanism of microtubule stabilization and motor regulation by MAP9.bioRxiv : the preprint server for biology · 2025Article
- Alzheimer's disease pathogenetic progression is associated with changes in regulated retained introns and editing of circular RNAs.Frontiers in molecular neuroscience · 2023Article
- Revisiting the multisite phosphorylation that produces the M-phase supershift of key mitotic regulators.Molecular biology of the cell · 2022Article
- Transcriptomic profile of leg muscle during early growth and development in Haiyang yellow chicken.Archives animal breeding · 2021Article
- Enhanced expression of microtubule-associated protein 7 functioned as a contributor to cervical cancer cell migration and is predictive of adverse prognosis.Cancer cell international · 2020Article
- A Cell Junctional Protein Network Associated with Connexin-26.International journal of molecular sciences · 2018Article
- Article
- Two-step interphase microtubule disassembly aids spindle morphogenesis.BMC biology · 2018Article
- ReMAPping the microtubule landscape: How phosphorylation dictates the activities of microtubule-associated proteins.Developmental dynamics : an official publication of the American Association of Anatomists · 2018Review
- Tau-based fluorescent protein fusions to visualize microtubules.Cytoskeleton (Hoboken, N.J.) · 2017Article
- DCLK1 phosphorylates the microtubule-associated protein MAP7D1 to promote axon elongation in cortical neurons.Developmental neurobiology · 2017Article
- The identification of novel targets of miR-16 and characterization of their biological functions in cancer cells.Molecular cancer · 2013Article
- Tubulin polymerization promoting protein 1 (Tppp1) phosphorylation by Rho-associated coiled-coil kinase (rock) and cyclin-dependent kinase 1 (Cdk1) inhibits microtubule dynamics to increase cell proliferation.The Journal of biological chemistry · 2013Article
- Drugs that target dynamic microtubules: a new molecular perspective.Medicinal research reviews · 2011Review
- The expression ratio of Map7/B2M is prognostic for survival in patients with stage II colon cancer.International journal of oncology · 2008Article
- Conservation of intrinsic disorder in protein domains and families: II. functions of conserved disorder.Journal of proteome research · 2006Article
- Microtubule regulation in mitosis: tubulin phosphorylation by the cyclin-dependent kinase Cdk1.Molecular biology of the cell · 2006Article
- Phosphorylation controls CLIMP-63-mediated anchoring of the endoplasmic reticulum to microtubules.Molecular biology of the cell · 2005Article
- Analysis of the adenovirus E1B-55K-anchored proteome reveals its link to ubiquitination machinery.Journal of virology · 2002Article
- FEZ1/LZTS1 gene at 8p22 suppresses cancer cell growth and regulates mitosis.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression levels of E-MAP-115, a microtubule-associated protein that stabilizes microtubules, increase with epithelial cell polarization and differentiation (Masson and Kreis, 1993). Although polarizing cells contain significant amounts of this protein, they can still divide and thus all stabilized microtubules must disassemble at the onset of mitosis to allow formation of the dynamic mitotic spindle. We show here that binding of E-MAP-115 to microtubules is regulated by phosphorylation during the cell cycle. Immunolabeling of HeLa cells for E-MAP-115 indicates that the protein is absent from microtubules during early prophase and progressively reassociates with microtubules after late prophase. A fraction of E-MAP-115 from HeLa cells released from a block at the G1/S boundary runs with higher apparent molecular weight on SDS-PAGE, with a peak correlating with the maximal number of cells in early stages of mitosis. E-MAP-115 from nocodazole-arrested mitotic cells, which can be obtained in larger amounts, displays identical modifications and was used for further biochemical characterization. The level of incorporation of 32P into mitotic E-MAP-115 is about 15-fold higher than into the interphase protein. Specific threonine phosphorylation occurs in mitosis, and the amount of phosphate associated with serine also increases. Hyperphosphorylated E-MAP-115 from mitotic cells cannot bind stably to microtubules in vitro. These results suggest that phosphorylation of E-MAP-115 is a prerequisite for increasing the dynamic properties of the interphase microtubules which leads to the assembly of the mitotic spindle at the onset of mitosis. Microtubule-associated proteins are thus most likely key targets for kinases which control changes in microtubule dynamic properties at the G2- to M-phase transition.
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