ArticleThe EMBO journal1995
Generation of a human X-derived minichromosome using telomere-associated chromosome fragmentation.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Review
- Biocircuits in plants and eukaryotic algae.Frontiers in plant science · 2022Review
- Assembly of Multiple Full-Size Genes or Genomic DNA Fragments on Human Artificial Chromosomes Using the Iterative Integration System.Current protocols · 2021Article
- Analysis of Complex DNA Rearrangements during Early Stages of HAC Formation.ACS synthetic biology · 2020Article
- Gene expressing human artificial chromosome vectors: Advantages and challenges for gene therapy.Experimental cell research · 2020Review
- Article
- Alpha satellite DNA biology: finding function in the recesses of the genome.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2018Review
- Review
- Genetic and epigenetic regulation of centromeres: a look at HAC formation.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- De novo formed satellite DNA-based mammalian artificial chromosomes and their possible applications.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Article
- Article
- Histone modifications within the human X centromere region.PloS one · 2009Article
- Integration-deficient lentiviral vectors: a slow coming of age.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Review
- Flow analysis and sorting of microchromosomes (<3 Mb).Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2007Article
- Construction and behavior of engineered minichromosomes in maize.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Telomere-mediated chromosomal truncation in maize.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Increased missegregation and chromosome loss with decreasing chromosome size in vertebrate cells.Chromosoma · 2006Article
- Human artificial chromosome vectors meet stem cells: new prospects for gene delivery.Stem cell reviews · 2006Review
- The manipulation of chromosomes by mankind: the uses of microcell-mediated chromosome transfer.Chromosoma · 2005Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A linear mammalian artificial chromosome vector will require at least three functional elements: a centromere, two telomeres and replication origins. One route to generate such a vector is by the fragmentation of an existing chromosome. We have previously described the use of cloned telomeric DNA to generate and stably rescue truncated derivatives of a human X chromosome in a somatic cell hybrid. Further rounds of telomere-associated chromosome fragmentation have now been used to engineer a human X-derived minichromosome. This minichromosome is estimated to be < 10 Mb in size. In situ hybridization and molecular analysis reveal that the minichromosome has a linear structure, with two introduced telomere constructs flanking a 2.5 Mb alpha-satellite array. The highly truncated chromosome also retains some chromosome-specific DNA, originating from Xp11.21. There is no significant change in the mitotic stability of the minichromosome as compared with the X chromosome from which it was derived.
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