ArticleThe EMBO journal1995
The N-terminal domain of human DNA ligase I contains the nuclear localization signal and directs the enzyme to sites of DNA replication.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 78 citations in OpenAlex.
- Probing the mechanism of nick searching by LIG1 at the single-molecule level.Nucleic acids research · 2024Article
- Altered DNA ligase activity in human disease.Mutagenesis · 2020Review
- The oncogenic RNA-binding protein SRSF1 regulates LIG1 in non-small cell lung cancer.Laboratory investigation; a journal of technical methods and pathology · 2018Article
- Review
- DNA ligases as therapeutic targets.Translational cancer research · 2013Article
- Functional redundancy between DNA ligases I and III in DNA replication in vertebrate cells.Nucleic acids research · 2012Article
- DNA ligase I, the replicative DNA ligase.Sub-cellular biochemistry · 2012Review
- Eukaryotic DNA ligases: structural and functional insights.Annual review of biochemistry · 2008Review
- Distribution of DNA replication proteins in Drosophila cells.BMC cell biology · 2007Article
- Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation.Nucleic acids research · 2007Article
- Differential recruitment of DNA Ligase I and III to DNA repair sites.Nucleic acids research · 2006Article
- PCNA acts as a stationary loading platform for transiently interacting Okazaki fragment maturation proteins.Nucleic acids research · 2005Article
- A pRb-independent mechanism preserves the postmitotic state in terminally differentiated skeletal muscle cells.The Journal of cell biology · 2004Article
- Modular structure of the human lamin B2 replicator.Molecular and cellular biology · 2004Article
- Functional analysis of subcellular localization and protein-protein interaction sequences in the essential DNA ligase I protein of fission yeast.Nucleic acids research · 2004Article
- DNA replication: a complex matter.EMBO reports · 2003Review
- Cell cycle-dependent dynamic association of cyclin/Cdk complexes with human DNA replication proteins.The EMBO journal · 2002Article
- Etoposide induces the dispersal of DNA ligase I from replication factories.Molecular biology of the cell · 2001Article
- Evidence for a replication function of FFA-1, the Xenopus orthologue of Werner syndrome protein.The Journal of cell biology · 2001Article
- Domain structure, localization, and function of DNA polymerase eta, defective in xeroderma pigmentosum variant cells.Genes & development · 2001Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA replication in mammalian cells occurs in discrete nuclear foci called 'replication factories'. Here we show that DNA ligase I, the main DNA ligase activity in proliferating cells, associates with the factories during S phase but displays a diffuse nucleoplasmic distribution in non-S phase nuclei. Immunolocalization analysis of both chloramphenicol acetyltransferase (CAT)-DNA ligase I fusion proteins and epitope tagged DNA ligase I mutants allowed the identification of a 13 amino acid functional nuclear localization signal (NLS) located in the N-terminal regulatory domain of the protein. Furthermore, the NLS is immediately preceded by a 115 amino acid region required for the association of the enzyme with the replication factories. We propose that in vivo the activity of DNA ligase I could be modulated through the control of its sub-nuclear compartmentalization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.