ArticleThe EMBO journal1995
Extending dimerization interfaces: the bZIP basic region can form a coiled coil.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 102 citations in OpenAlex.
- BPabZIP, a new bZIP protein motif that promotes binding near, and displacement of, nucleosomes.bioRxiv : the preprint server for biology · 2026Article
- The transition state for coupled folding and binding of a disordered DNA binding domain resembles the unbound state.Nucleic acids research · 2024Article
- Review
- Selective antagonism of cJun for cancer therapy.Journal of experimental & clinical cancer research : CR · 2020Review
- CREB Family Transcription Factors Are Major Mediators of BDNF Transcriptional Autoregulation in Cortical Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Dominant-Negative ATF5 Compromises Cancer Cell Survival by Targeting CEBPB and CEBPD.Molecular cancer research : MCR · 2020Article
- Transient Delivery of A-C/EBP Protein Perturbs Differentiation of 3T3-L1 Cells and Induces Preadipocyte Marker Genes.Frontiers in molecular biosciences · 2020Article
- Selection of Protein-Protein Interactions of Desired Affinities with a Bandpass Circuit.Journal of molecular biology · 2019Article
- A-ZIP53, a dominant negative reveals the molecular mechanism of heterodimerization between bZIP53, bZIP10 and bZIP25 involved in Arabidopsis seed maturation.Scientific reports · 2017Article
- RUNX1-ETO and RUNX1-EVI1 Differentially Reprogram the Chromatin Landscape in t(8;21) and t(3;21) AML.Cell reports · 2017Article
- Role of DNA binding sites and slow unbinding kinetics in titration-based oscillators.Physical review. E, Statistical, nonlinear, and soft matter physics · 2015Article
- Increasing the affinity of selective bZIP-binding peptides through surface residue redesign.Protein science : a publication of the Protein Society · 2014Article
- Networks of bZIP protein-protein interactions diversified over a billion years of evolution.Science (New York, N.Y.) · 2013Article
- P6981, an arylstibonic acid, is a novel low nanomolar inhibitor of cAMP response element-binding protein binding to DNA.Molecular pharmacology · 2012Article
- Ubiquitin tagged dominant negative induces degradation of B-ZIP proteins.Biochemical and biophysical research communications · 2012Article
- Regulated ATF5 loss-of-function in adult mice blocks formation and causes regression/eradication of gliomas.Oncogene · 2012Article
- Suppression of the C/EBP family of transcription factors in adipose tissue causes lipodystrophy.Journal of molecular endocrinology · 2011Article
- Design of peptide inhibitors that bind the bZIP domain of Epstein-Barr virus protein BZLF1.Journal of molecular biology · 2011Article
- Article
- 12 Arylstibonic acids that inhibit the DNA binding of five B-ZIP dimers.Journal of structural biology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We appended a rationally designed acidic amphipathic protein sequence to the N-terminus of a leucine zipper. Circular dichroism data indicate that this engineered polypeptide sequence can 'zipper' up the basic region of a bZIP monomer into a heterodimeric coiled coil. This propagation of the leucine zipper dimerization interface into the basic region can proceed for up to four heptads and stabilizes the heterodimer complex 2.5 kcal/mol or > 100-fold. The acidic nature of the extension is the most critical component of the design, suggesting that the extension is acting as a DNA mimetic. The dimerization prevents the basic region in this heterodimeric coiled coil structure from binding to DNA. Gel-shift, fluorescence and transient transfection assays indicate that the acidic extension appended to a leucine zipper can inactivate the DNA-binding and transactivation properties of the bZIP protein C/EBP. The three bZIP basic regions examined in this study dimerize with similar stability with the acidic extension, suggesting that this N-terminal extension can be used to develop dominant-negatives to other bZIP transcription factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.