ArticleThe Biochemical journal1995
The novel cyclic dinucleotide 3'-5' cyclic diguanylic acid binds to p21ras and enhances DNA synthesis but not cell replication in the Molt 4 cell line.
Article in The Biochemical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Integrated multiomics reveals starvation-driven keratin degradation and persistence iniScience · 2026Article
- Impact and applications of cyclic di-GMP second messenger signaling in biotechnology and medicine.Sustainable microbiology · 2025Review
- Bacterial c-di-GMP triggers metamorphosis of mussel larvae through a STING receptor.NPJ biofilms and microbiomes · 2024Article
- Nucleotides as Bacterial Second Messengers.Molecules (Basel, Switzerland) · 2023Review
- Association of Biofilm Inducer withArchives of Razi Institute · 2022Article
- Yin and Yang of Biofilm Formation and Cyclic di-GMP Signaling of the Gastrointestinal Pathogen Salmonella enterica Serovar Typhimurium.Journal of innate immunity · 2022Review
- Biofilm formation - what we can learn from recent developments.Journal of internal medicine · 2018Article
- Discovery of the Second Messenger Cyclic di-GMP.Methods in molecular biology (Clifton, N.J.) · 2017Review
- Diversity of Cyclic Di-GMP-Binding Proteins and Mechanisms.Journal of bacteriology · 2016Review
- OAS proteins and cGAS: unifying concepts in sensing and responding to cytosolic nucleic acids.Nature reviews. Immunology · 2014Review
- Responses to elevated c-di-GMP levels in mutualistic and pathogenic plant-interacting bacteria.PloS one · 2014Article
- Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.Microbiology and molecular biology reviews : MMBR · 2013Review
- Sensing the messenger: the diverse ways that bacteria signal through c-di-GMP.Protein science : a publication of the Protein Society · 2012Review
- SagS contributes to the motile-sessile switch and acts in concert with BfiSR to enable Pseudomonas aeruginosa biofilm formation.Journal of bacteriology · 2011Article
- Connecting quorum sensing, c-di-GMP, pel polysaccharide, and biofilm formation in Pseudomonas aeruginosa through tyrosine phosphatase TpbA (PA3885).PLoS pathogens · 2009Article
- BdlA, a chemotaxis regulator essential for biofilm dispersion in Pseudomonas aeruginosa.Journal of bacteriology · 2006Article
- Control of formation and cellular detachment from Shewanella oneidensis MR-1 biofilms by cyclic di-GMP.Journal of bacteriology · 2006Article
- Phenotypic convergence mediated by GGDEF-domain-containing proteins.Journal of bacteriology · 2005Article
- Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The effect of the novel, naturally occurring nucleotide 3'-5' cyclic diguanylic acid (c-di-GMP) on the lymphoblastoid Molt 4 cell line was studied. When exposed to this guanine nucleotide. Molt 4 cells exhibited a marked increase in [3H]thymidine incorporation, up to 200-fold at 50 microM c-di-GMP. Correspondingly, the DNA content of the treated cells was 9-fold higher than untreated cells. Stimulation of [3H]thymidine incorporation into the cells was time- and concentration-dependent. This effect was specific and was not observed with GMP or cyclic GMP, nor with the unhydrolysable GTP analogues, guanosine 5'-[gamma-thio]triphosphate and guanosine 5'-[beta gamma-imido]-triphosphate. C-di-GMP entrance into the cells was experimentally verified and occurred without using any means of cell permeabilization. SDS/PAGE analysis of cells exposed to [32P]c-di-GMP, followed by autoradiography, revealed the labelling of three low-molecular-mass proteins at 18-27 kDa. The labelling is highly specific to c-di-GMP and its extent was not affected by other guanine nucleotides. 2. One of the c-di-GMP-binding proteins was found to be the p21ras protein, by immunoprecipitation with the anti-Ras monoclonal antibody Y13-259. The effects described appear to be unique for c-di-GMP and, taken together, raise the possibility that an irreversible binding of this guanine nucleotide to the growth-promoting p21ras protein results in a fixed active conformation of this protein affecting DNA synthesis. Strikingly, although at 48 h of growth markedly high DNA levels were found in Molt 4 cells treated with c-di-GMP, this guanine nucleotide had no effect on cell replication during this period. Thus Molt 4 cells exposed to c-di-GMP enter the S phase uncoordinated with their overall replication rate.
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