ArticleThe Biochemical journal1995
Identification of a heparin-binding protein using monoclonal antibodies that block heparin binding to porcine aortic endothelial cells.
Article in The Biochemical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
- Article
- Endothelial nitric oxide synthase activation is required for heparin receptor effects on vascular smooth muscle cells.American journal of physiology. Cell physiology · 2020Article
- Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity.Journal of visualized experiments : JoVE · 2017Article
- Transmembrane Protein 184A Is a Receptor Required for Vascular Smooth Muscle Cell Responses to Heparin.The Journal of biological chemistry · 2016Article
- Heparin Decreases in Tumor Necrosis Factor α (TNFα)-induced Endothelial Stress Responses Require Transmembrane Protein 184A and Induction of Dual Specificity Phosphatase 1.The Journal of biological chemistry · 2016Article
- Heparin responses in vascular smooth muscle cells involve cGMP-dependent protein kinase (PKG).Journal of cellular physiology · 2014Article
- Hydrogel surfaces to promote attachment and spreading of endothelial progenitor cells.Journal of tissue engineering and regenerative medicine · 2013Article
- Bivalirudin in combination with heparin to control mesenchymal cell procoagulant activity.PloS one · 2012Article
- Heparin treatment of vascular smooth muscle cells results in the synthesis of the dual-specificity phosphatase MKP-1.Journal of cellular biochemistry · 2010Article
- Antiendothelial cell antibodies in vasculitis and connective tissue disease.Annals of the rheumatic diseases · 2006Review
- Vascular endothelial tissue factor pathway inhibitor kinetics in culture following exposure to DX-9065a--a selective and direct factor Xa inhibitor.Journal of thrombosis and thrombolysis · 2004Article
- A unique heparin-binding domain in the envelope protein of the neuropathogenic PVC-211 murine leukemia virus may contribute to its brain capillary endothelial cell tropism.Journal of virology · 2001Article
- Mast cell granule heparin proteoglycan induces lacunae in confluent endothelial cell monolayers.The American journal of pathology · 1999Article
- Microbial adherence to and invasion through proteoglycans.Infection and immunity · 1997Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The binding of heparin or heparan sulphate to a variety of cell types results in specific changes in cell function. Endothelial cells treated with heparin alter their synthesis of heparan sulphate proteoglycans and extracellular matrix proteins. In order to identify a putative endothelial cell heparin receptor that could be involved in heparin signalling, anti-(endothelial cell) monoclonal antibodies that significantly inhibit heparin binding to endothelial cells were prepared. Four of these antibodies were employed in affinity-chromatographic isolation of a heparin-binding protein from detergent-solubilized endothelial cells. The heparin-binding protein isolated from porcine aortic endothelial cells using four different monoclonal antibodies has an M(r) of 45,000 assessed by SDS/PAGE. The 45,000-M(r) heparin-binding polypeptide is isolated as a multimer. The antibody-isolated protein binds to heparin-affinity columns as does the pure 45,000-M(r) polypeptide, consistent with its identification as a putative endothelial heparin receptor.
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Registered trials
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