ArticleApplied and environmental microbiology1995
Production of Trichoderma reesei cellulases on glucose-containing media.
Article in Applied and environmental microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Enhanced heterologous gene expression in Trichoderma reesei by promoting multicopy integration.Applied microbiology and biotechnology · 2024Article
- Overexpression of the Transcription Factor Azf1 Reveals Novel Regulatory Functions and Impacts β-Glucosidase Production inJournal of fungi (Basel, Switzerland) · 2023Article
- Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei.Bioresources and bioprocessing · 2022Article
- Heterologous Expression of Secondary Metabolite Genes inJournal of fungi (Basel, Switzerland) · 2022Article
- Development of a powerful synthetic hybrid promoter to improve the cellulase system of Trichoderma reesei for efficient saccharification of corncob residues.Microbial cell factories · 2022Article
- An overview on current molecular tools for heterologous gene expression in Trichoderma.Fungal biology and biotechnology · 2021Review
- Assessment of the ptxD gene as a growth and selective marker in Trichoderma atroviride using Pccg6, a novel constitutive promoter.Microbial cell factories · 2020Article
- Novel genetic tools that enable highly pure protein production in Trichoderma reesei.Scientific reports · 2019Article
- Deletion of the small GTPaseFungal biology and biotechnology · 2019Article
- The Promoter Toolbox for Recombinant Gene Expression inFrontiers in bioengineering and biotechnology · 2018Review
- Self-induction system for cellulase production by cellobiose produced from glucose in Rhizopus stolonifer.Scientific reports · 2017Article
- Trichoderma reesei xylanase 5 is defective in the reference strain QM6a but functional alleles are present in other wild-type strains.Applied microbiology and biotechnology · 2017Article
- Mutagenesis of Trichoderma reesei endoglucanase I: impact of expression host on activity and stability at elevated temperatures.BMC biotechnology · 2015Article
- A constitutive expression system for glycosyl hydrolase family 7 cellobiohydrolases in Hypocrea jecorina.Biotechnology for biofuels · 2015Article
- Cost-effective lignocellulolytic enzyme production by Trichoderma reesei on a cane molasses medium.Biotechnology for biofuels · 2014Article
- Construction of a starch-inducible homologous expression system to produce cellulolytic enzymes from Acremonium cellulolyticus.Journal of industrial microbiology & biotechnology · 2013Article
- Achieving efficient protein expression in Trichoderma reesei by using strong constitutive promoters.Microbial cell factories · 2012Article
- A homologous production system for Trichoderma reesei secreted proteins in a cellulase-free background.Applied microbiology and biotechnology · 2012Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The filamentous fungus Trichoderma reesei was shown to secrete active cellobiohydrolase I and the endoglucanase I catalytic core domain into the culture medium when the fungus was grown on glucose-containing medium. The expression of the proteins was driven by the promoters of the elongation factor 1 alpha, tef1, and the unidentified gene for cDNA1. The cDNA1 promoter gave the best yields. The highest amounts of cellobiohydrolase I and the endoglucanase I core, being 50 to 100 mg/liter, accounted for more than half of the total protein secreted by the fungus. The levels obtained with the tef1 promoter were 20 to 50 times lower.
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