ArticleAntimicrobial agents and chemotherapy1995
Role of extracellular calcium in in vitro uptake and intraphagocytic location of macrolides.
Article in Antimicrobial agents and chemotherapy, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- Effect of single oral dose of azithromycin, clarithromycin, and roxithromycin on polymorphonuclear leukocyte function assessed ex vivo by flow cytometry.Antimicrobial agents and chemotherapy · 1996Trial
- Intracellular Penetration and Effects of Antibiotics onAntibiotics (Basel, Switzerland) · 2019Review
- Azithromycin inhibits IL-1 secretion and non-canonical inflammasome activation.Scientific reports · 2015Article
- Ketolides--the modern relatives of macrolides : the pharmacokinetic perspective.Clinical pharmacokinetics · 2009Review
- Cellular uptake and efflux of azithromycin, erythromycin, clarithromycin, telithromycin, and cethromycin.Antimicrobial agents and chemotherapy · 2005Article
- Role of leukocyte elastase in preventing cellular re-colonization of the mural thrombus.The American journal of pathology · 2004Article
- Interaction of the new ketolide ABT-773 (cethromycin) with human polymorphonuclear neutrophils and the phagocytic cell line PLB-985 in vitro.Antimicrobial agents and chemotherapy · 2004Article
- Influence of P-glycoprotein inhibitors on accumulation of macrolides in J774 murine macrophages.Antimicrobial agents and chemotherapy · 2003Article
- Involvement of the mural thrombus as a site of protease release and activation in human aortic aneurysms.The American journal of pathology · 2002Article
- Cellular uptake of two fluoroketolides, HMR 3562 and HMR 3787, by human polymorphonuclear neutrophils in vitro.Antimicrobial agents and chemotherapy · 2001Article
- Review
- Interference of antibacterial agents with phagocyte functions: immunomodulation or "immuno-fairy tales"?Clinical microbiology reviews · 2000Review
- The new ketolide HMR3647 accumulates in the azurophil granules of human polymorphonuclear cells.Antimicrobial agents and chemotherapy · 1999Article
- Mechanism underlying levofloxacin uptake by human polymorphonuclear neutrophils.Antimicrobial agents and chemotherapy · 1999Article
- Interactions between HMR 3647, a new ketolide, and human polymorphonuclear neutrophils.Antimicrobial agents and chemotherapy · 1998Article
- Cellular accumulation of the new ketolide RU 64004 by human neutrophils: comparison with that of azithromycin and roxithromycin.Antimicrobial agents and chemotherapy · 1997Article
- In vitro susceptibilities of Bartonella henselae, B. quintana, B. elizabethae, Rickettsia rickettsii, R. conorii, R. akari, and R. prowazekii to macrolide antibiotics as determined by immunofluorescent-antibody analysis of infected Vero cell monolayers.Antimicrobial agents and chemotherapy · 1997Article
- Intracellular activities of RP 59500 (quinupristin-dalfopristin) and sparfloxacin against Enterococcus faecium.Antimicrobial agents and chemotherapy · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We compared the uptakes and intracellular locations of four 14-membered-ring macrolides (roxithromycin, dirithromycin, erythromycin, and erythromycylamine) in human polymorphonuclear neutrophils (PMNs) in vitro. Intracellular location was assessed by cell fractionation and uptake kinetics in cytoplasts (granule-poor PMNs). Trapping of dirithromycin within PMN granules (up to 80% at 30 min) was significantly more marked than the intracellular trapping of the other drugs (erythromycylamine, 45% +/- 5.1%; erythromycin, 42% +/- 3.7%; roxithromycin, 35% +/- 3.0%). A new finding was that, in the absence of extracellular calcium, the uptakes of all of the macrolides by PMNs and cytoplasts were significantly impaired, by about 50% (PMN) and 90% (cytoplasts). Furthermore, inorganic Ca2+ channel blockers inhibited macrolide uptake in a concentration-dependent manner, with 50% inhibitory concentrations of 1.6 to 2.0 mM and 29 to 35 microM, respectively, for Ni2+ and La3+. The intracellular distributions of the drugs were unchanged in the presence of Ni2+ and La3+ and in Ca(2+)-free medium supplemented with ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid. The organic Ca2+ channel blocker nifedipine had no effect on macrolide uptake, whereas verapamil inhibited it in a time- and concentration-dependent manner. These data show the importance of extracellular Ca2+ in macrolide uptake by phagocytes and suggest a link with Ca2+ channels or a Ca2+ channel-operated mechanism.
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