ArticleThe American journal of pathology1995
In situ mRNA hybridization technique for analysis of metastasis-related genes in human colon carcinoma cells.
Article in The American journal of pathology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 85 citations in OpenAlex.
- Article
- Chemokines orchestrate tumor cells and the microenvironment to achieve metastatic heterogeneity.Cancer metastasis reviews · 2021Review
- Copy number and transcriptome alterations associated with metastatic lesion response to treatment in colorectal cancer.Clinical and translational medicine · 2021Article
- Collagen IV-conveyed signals can regulate chemokine production and promote liver metastasis.Oncogene · 2018Article
- E-cadherin: A potential biomarker of colorectal cancer prognosis.Oncology letters · 2017Article
- Molecular targets and pathways involved in liver metastasis of colorectal cancer.Clinical & experimental metastasis · 2015Review
- Article
- A truncated isoform of the protein phosphatase 2A B56gamma regulatory subunit may promote genetic instability and cause tumor progression.The American journal of pathology · 2003Article
- Multiparametric in situ mRNA hybridization analysis of gastric biopsies predicts lymph node metastasis in patients with gastric carcinoma.Japanese journal of cancer research : Gann · 2002Article
- In situ mRNA hybridization technique for analysis of human telomerase RNA in gastric precancerous and cancerous lesions.Japanese journal of cancer research : Gann · 1998Article
- Alterative expression of the collagenase and adhesion molecules in the highly metastatic clones of human colonic cancer cell lines.Clinical & experimental metastasis · 1998Article
- Effect of host microenvironment on the microcirculation of human colon adenocarcinoma.The American journal of pathology · 1997Article
- Correlation of metastasis-related gene expression with metastatic potential in human prostate carcinoma cells implanted in nude mice using an in situ messenger RNA hybridization technique.The American journal of pathology · 1997Article
- Technical considerations for studying cancer metastasis in vivo.Clinical & experimental metastasis · 1997Review
- Multiparametric in situ mRNA hybridization analysis to predict disease recurrence in patients with colon carcinoma.The American journal of pathology · 1996Article
- Suppression of angiogenesis and therapy of human colon cancer liver metastasis by systemic administration of interferon-alpha.Neoplasia (New York, N.Y.)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The purpose of this study was to determine whether the expression level of several genes that regulate different steps of the metastatic process correlates with the metastatic potential of human colon carcinoma cells. The mRNA expression level for epidermal growth factor receptor (growth), basic fibroblast growth factor and interleukin-8 (angiogenesis), type IV collagenase (invasion), E-cadherin and carcinoembryonic antigen (adhesion), and the multidrug resistance gene mdr-1 (drug resistance) in the human KM12 colon carcinoma cell lines and clones with different metastatic potential was measured by Northern blot analysis and by in situ hybridization technique. Highly metastatic KM12SM and KM1214 cells growing in culture uniformly expressed high levels of epidermal growth factor receptor, basic fibroblast growth factor, and carcinoembryonic antigen mRNA, whereas cultures of low metastatic KM12C, clone 1, clone 3, and clone 6 cells displayed heterogeneous patterns of expression. KM12C (low metastatic) and KM12SM (highly metastatic) cells were implanted into the subcutis (ectopic) or the wall of the cecum (orthotopic) of nude mice. The mRNA expression level for epidermal growth factor receptor, basic fibroblast growth factor, interleukin-8, type IV collagenase, carcinoembryonic antigen, and mdr-1 was increased in the cecal wall tumors as compared with subcutaneous tumors or in vitro cultures. These data demonstrate a direct correlation between constitutive and inducible expression of several metastasis-related genes and the metastatic potential of human colon carcinoma cells.
Indexed as
Identifiers
7485388PMC1869504W2397228914What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.