ArticleAmerican journal of human genetics1995
Toward fully automated genotyping: genotyping microsatellite markers by deconvolution.
Article in American journal of human genetics, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed.
- Evaluation of the Effectiveness of Single-Nucleotide Polymorphisms Versus Microsatellites for Parentage Verification in Horse Breeds.Veterinary sciences · 2025Article
- Deciphering the Genetic Diversity of Landraces With High-Throughput SNP Genotyping of DNA Bulks: Methodology and Application to the Maize 50k Array.Frontiers in plant science · 2020Article
- New York State TrueAllele ® casework validation study.Journal of forensic sciences · 2013Article
- Next-generation phenotyping: requirements and strategies for enhancing our understanding of genotype-phenotype relationships and its relevance to crop improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Review
- HighSSR: high-throughput SSR characterization and locus development from next-gen sequencing data.Bioinformatics (Oxford, England) · 2012Article
- Analysis of phenotypic and microsatellite-based diversity of maize landraces in India, especially from the north east Himalayan region.Genetica · 2010Article
- An information gap in DNA evidence interpretation.PloS one · 2009Article
- A new approach to characterize populations of Schistosoma mansoni from humans: development and assessment of microsatellite analysis of pooled miracidia.Tropical medicine & international health : TM & IH · 2009Article
- In vitro direct repeats-mediated deletion during PCR amplification.Molecular biotechnology · 2008Article
- Dopaminergic mutations: within-family association and linkage in multiplex alcohol dependence families.American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics · 2008Article
- Evaluation of DNA pooling for the estimation of microsatellite allele frequencies: a case study using striped bass (Morone saxatilis).Genetics · 2006Article
- Automated DNA profile analysis.Forensic science, medicine, and pathology · 2005Article
- PPC: an algorithm for accurate estimation of SNP allele frequencies in small equimolar pools of DNA using data from high density microarrays.Nucleic acids research · 2005Article
- Estimating haplotype frequencies in pooled DNA samples when there is genotyping error.BMC genetics · 2005Article
- A genome wide search for alcoholism susceptibility genes.American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics · 2004Article
- Article
- A novel method for automatic genotyping of microsatellite markers based on parametric pattern recognition.Human genetics · 2003Article
- Novel amplification of DNA in a hairpin structure: towards a radical elimination of PCR errors from amplified DNA.Nucleic acids research · 2003Article
- Toward high-throughput genotyping: dynamic and automatic software for manipulating large-scale genotype data using fluorescently labeled dinucleotide markers.Genome research · 2001Article
- Pooled genotyping of microsatellite markers in parent-offspring trios.Genome research · 2000Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Dense genetic linkage maps have been constructed for the human and mouse genomes, with average densities of 2.9 cM and 0.35 cM, respectively. These genetic maps are crucial for mapping both Mendelian and complex traits and are useful in clinical genetic diagnosis. Current maps are largely comprised of abundant, easily assayed, and highly polymorphic PCR-based microsatellite markers, primarily dinucleotide (CA)n repeats. One key limitation of these length polymorphisms is the PCR stutter (or slippage) artifact that introduces additional stutter bands. With two (or more) closely spaced alleles, the stutter bands overlap, and it is difficult to accurately determine the correct alleles; this stutter phenomenon has all but precluded full automation, since a human must visually inspect the allele data. We describe here novel deconvolution methods for accurate genotyping that mathematically remove PCR stutter artifact from microsatellite markers. These methods overcome the manual interpretation bottleneck and thereby enable full automation of genetic map construction and use. New functionalities, including the pooling of DNAs and the pooling of markers, are described that may greatly reduce the associated experimentation requirements.
Indexed as
Identifiers
7485172PMC1801391What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.