ArticlePlant physiology1995
Intracellular carbonic anhydrase of Chlamydomonas reinhardtii.
Article in Plant physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 100 citations in OpenAlex.
- Sensitivity of Photosystem II to Photoinhibition inInternational journal of molecular sciences · 2026Article
- Super-resolution expansion microscopy reveals nanoscale protein domains and CObioRxiv : the preprint server for biology · 2026Article
- Highly Active Carbonic Anhydrase of the Thylakoid Lumen ofPlants (Basel, Switzerland) · 2024Article
- CAH3 fromCells · 2024Review
- α-CAs from Photosynthetic Organisms.International journal of molecular sciences · 2022Review
- Advances in understanding the physiological role and locations of carbonic anhydrases in C3 plant cells.Protoplasma · 2021Review
- Carbonic Anhydrases in Photosynthesizing Cells of C3 Higher Plants.Metabolites · 2019Review
- Expression of seven carbonic anhydrases in red alga Gracilariopsis chorda and their subcellular localization in a heterologous system, Arabidopsis thaliana.Plant cell reports · 2019Article
- An Update on the Metabolic Roles of Carbonic Anhydrases in the Model Alga Chlamydomonas reinhardtii.Metabolites · 2018Review
- Regulation of CCM genes in Chlamydomonas reinhardtii during conditions of light-dark cycles in synchronous cultures.Plant molecular biology · 2014Article
- Phosphorylation controls the localization and activation of the lumenal carbonic anhydrase in Chlamydomonas reinhardtii.PloS one · 2012Article
- The carbonic anhydrase isoforms of Chlamydomonas reinhardtii: intracellular location, expression, and physiological roles.Photosynthesis research · 2011Review
- Inorganic carbon acquisition by eukaryotic algae: four current questions.Photosynthesis research · 2010Review
- Proposed carbon dioxide concentrating mechanism in Chlamydomonas reinhardtii.Eukaryotic cell · 2007Review
- Carbonic anhydrase activities in pea thylakoids.Photosynthesis research · 2004Article
- The Chlamydomonas reinhardtii cia3 mutant lacking a thylakoid lumen-localized carbonic anhydrase is limited by CO2 supply to rubisco and not photosystem II function in vivo.Plant physiology · 2003Article
- Historical perspective on microalgal and cyanobacterial acclimation to low- and extremely high-CO(2) conditions.Photosynthesis research · 2003Article
- Extrinsic photosystem II carbonic anhydrase in maize mesophyll chloroplasts.Plant physiology · 2002Article
- Evidence for an inorganic carbon-concentrating mechanism in the symbiotic dinoflagellate Symbiodinium sp.Plant physiology · 1999Article
- Isolation and characterization of a mutant protoporphyrinogen oxidase gene from Chlamydomonas reinhardtii conferring resistance to porphyric herbicides.Plant molecular biology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An intracellular carbonic anhydrase (CA; EC 4.2.1.1) was purified to homogeneity from a mutant strain of Chlamydomonas reinhardtii (CW 92) lacking a cell wall. Intact cells were washed to remove periplasmic CA and were lysed and fractionated into soluble and membrane fractions by sedimentation. All of the CA activity sedimented with the membrane fraction and was dissociated by treatment with a buffer containing 200 mM KCI. Solubilized proteins were fractionated by ammonium sulfate precipitation, anionic exchange chromatography, and hydrophobic interaction chromatography. The resulting fraction had a specific activity of 1260 Wilbur-Anderson units/mg protein and was inhibited by acetazolamide (50% inhibition concentration, 12 nM). Final purification was accomplished by the specific absorption of the enzyme to a Centricon-10 microconcentrator filter. A single, 29.5-kD polypeptide was eluted from the filter with sodium dodecyl sulfate-polyacrylamide gel electrophoresis sample buffer, and a 1.5 M ammonium sulfate eluate contained CA activity. In comparison with human CA isoenzyme II, the N-terminal and internal amino acid sequences from the 29.5-kD polypeptide were 40% identical with the N-terminal region and 67% identical with an internal conserved region. Based on this evidence, we postulate that the 29.5-kD polypeptide is an internal CA in C. reinhardtii and that the enzyme is closely related to the alpha-type CAs observed in animal species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.