ArticleProceedings of the National Academy of Sciences of the United States of America1995
The activation domain of GAL4 protein mediates cooperative promoter binding with general transcription factors in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Distinct functions of three chromatin remodelers in activator binding and preinitiation complex assembly.PLoS genetics · 2022Article
- Transcriptomic Analysis of Resistant and Wild-Type Isolates Revealed Fludioxonil as a Candidate for Controlling the Emerging Isoprothiolane Resistant Populations ofFrontiers in microbiology · 2022Article
- Budding off: bringing functional genomics to Candida albicans.Briefings in functional genomics · 2016Review
- Article
- New and Redesigned pRS Plasmid Shuttle Vectors for Genetic Manipulation of Saccharomycescerevisiae.G3 (Bethesda, Md.) · 2012Article
- Mechanism of origin activation by monomers of R6K-encoded pi protein.Journal of molecular biology · 2007Article
- Single-molecule and population probing of chromatin structure using DNA methyltransferases.Methods (San Diego, Calif.) · 2007Article
- Gene expression from random libraries of yeast promoters.Genetics · 2006Article
- Conservation and evolution of cis-regulatory systems in ascomycete fungi.PLoS biology · 2004Article
- Regions of GAL4 critical for binding to a promoter in vivo revealed by a visual DNA-binding analysis.The EMBO journal · 2003Article
- GCN5 dependence of chromatin remodeling and transcriptional activation by the GAL4 and VP16 activation domains in budding yeast.Molecular and cellular biology · 2001Article
- Are all DNA binding and transcription regulation by an activator physiologically relevant?Molecular and cellular biology · 2001Article
- TATA element recognition by the TATA box-binding protein has been conserved throughout evolution.Genes & development · 1999Article
- Chromatin opening and transactivator potentiation by RAP1 in Saccharomyces cerevisiae.Molecular and cellular biology · 1999Article
- Requirements for chromatin modulation and transcription activation by the Pho4 acidic activation domain.Molecular and cellular biology · 1998Article
- SWI-SNF complex participation in transcriptional activation at a step subsequent to activator binding.Molecular and cellular biology · 1998Article
- Gal4p-mediated chromatin remodeling depends on binding site position in nucleosomes but does not require DNA replication.Molecular and cellular biology · 1998Article
- Activation domain-mediated enhancement of activator binding to chromatin in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Quantitation of putative activator-target affinities predicts transcriptional activating potentials.The EMBO journal · 1996Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Most proteins that activate RNA polymerase II-mediated transcription in eukaryotic cells contain sequence-specific DNA-binding domains and "activation" regions. The latter bind general transcription factors and/or coactivators and are required for high-level transcription. Their function in vivo is unknown. Since several activation domains bind the TATA-binding protein (TBP), TBP-associated factors, or other general factors in vitro, one role of the activation domain may be to facilitate promoter occupancy by supporting cooperative binding of the activator and general transcription factors. Using the GAL4 system of yeast, we have tested this model in vivo. It is demonstrated that the presence of a TATA box (the TBP binding site) facilitates binding of GAL4 protein to low- and moderate-affinity sites and that the activation domain modulates these effects. These results support the cooperative binding model for activation domain function in vivo.
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