ArticleProceedings of the National Academy of Sciences of the United States of America1995
Proteolytic maturation of protein C upon engineering the mouse mammary gland to express furin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 60 citations in OpenAlex.
- Beyond Processing: Furin as a Central Hub in Viral Pathogenesis and Genetic Susceptibility.Biomolecules · 2025Review
- Thrombin activation of protein C requires prior processing by a liver proprotein convertase.The Journal of biological chemistry · 2017Article
- Engineering protein processing of the mammary gland to produce abundant hemophilia B therapy in milk.Scientific reports · 2015Article
- Secretion of human protein C in mouse milk.International journal of molecular sciences · 2015Article
- Comparative study of the binding pockets of mammalian proprotein convertases and its implications for the design of specific small molecule inhibitors.International journal of biological sciences · 2010Article
- Expression of full-length human pro-urokinase in mammary glands of transgenic mice.Transgenic research · 2009Article
- Production of pharmaceutical proteins by transgenic animals.Comparative immunology, microbiology and infectious diseases · 2009Review
- Modeling familial British dementia in transgenic mice.Brain pathology (Zurich, Switzerland) · 2006Review
- Use of transgenic animals to improve human health and animal production.Reproduction in domestic animals = Zuchthygiene · 2005Review
- Biomedical and agricultural applications of animal transgenesis.Molecular biotechnology · 2004Review
- Transgenic mice expressing recombinant human protein C exhibit defects in lactation and impaired mammary gland development.Transgenic research · 2003Article
- Engineering immunity in the mammary gland.Journal of mammary gland biology and neoplasia · 2002Review
- Mammary gland immunity and mastitis susceptibility.Journal of mammary gland biology and neoplasia · 2002Review
- Transgenic animal bioreactors.Transgenic research · 2000Review
- The changing role of cell culture in the generation of transgenic livestock.Cytotechnology · 1999Article
- Production of recombinant human type I procollagen homotrimer in the mammary gland of transgenic mice.Transgenic research · 1999Article
- The mammary gland as a bioreactor: expression, processing, and production of recombinant proteins.Journal of mammary gland biology and neoplasia · 1998Review
- Furin: a mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteins.The Biochemical journal · 1997Review
- Phenotypic and genotypic stability of multiple lines of transgenic pigs expressing recombinant human protein C.Transgenic research · 1997Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endoproteolytic processing of the human protein C (HPC) precursor to its mature form involves cleavage of the propeptide after amino acids Lys-2-Arg-1 and removal of a Lys156-Arg157 dipeptide connecting the light and heavy chains. This processing was inefficient in the mammary gland of transgenic mice and pigs. We hypothesized that the protein processing capacity of specific animal organs may be improved by the coexpression of selected processing enzymes. We tested this by targeting expression of the human proprotein processing enzyme, named paired basic amino acid cleaving enzyme (PACE)/furin, or an enzymatically inactive mutant, PACEM, to the mouse mammary gland. In contrast to mice expressing HPC alone, or to HPC/PACEM bigenic mice, coexpression of PACE with HPC resulted in efficient conversion of the precursor to mature protein, with cleavage at the appropriate sites. These results suggest the involvement of PACE in the processing of HPC in vivo and represent an example of the engineering of animal organs into bioreactors with enhanced protein processing capacity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.