ArticleProceedings of the National Academy of Sciences of the United States of America1995
Contraction due to microtubule disruption is associated with increased phosphorylation of myosin regulatory light chain.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
74 citing papers in PubMed, 228 citations in OpenAlex.
- Paclitaxel induces NM2-dependent cellular contraction through GEF-H1 dissociation from microtubules and RhoA/ROCK activation in cancer cells.Molecular oncology · 2026Article
- Differential Cytoskeletal Control of Cell-Collagen Mechanics in High- and Low-Flow Glaucomatous Trabecular Meshwork Cells.ACS applied materials & interfaces · 2026Article
- A conserved VPS34-PIKfyve-TRPML1-myosin II axis regulates the speed of amoeboid cell migration.EMBO reports · 2026Article
- Tension anisotropy drives fibroblast phenotypic transition by self-reinforcing cell-extracellular matrix mechanical feedback.Nature materials · 2025Article
- Vimentin is a key regulator of cell mechanosensing through opposite actions on actomyosin and microtubule networks.Communications biology · 2024Article
- The role of mechanics in axonal stability and development.Seminars in cell & developmental biology · 2023Review
- A Novel Method in Identifying Pyroptosis and Apoptosis Based on the Double Resonator Piezoelectric Cytometry Technology.Biosensors · 2023Article
- Two nondimensional parameters for characterizing the nuclear morphology.Biophysical journal · 2021Article
- Microtubules control nuclear shape and gene expression during early stages of hematopoietic differentiation.The EMBO journal · 2020Article
- Article
- Glycoproteome in silkworm Bombyx mori and alteration by BmCPV infection.Journal of proteomics · 2020Article
- Nuclear Mechanics within Intact Cells Is Regulated by Cytoskeletal Network and Internal Nanostructures.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- Go with the flow: GEF-H1 mediated shear stress mechanotransduction in neutrophils.Small GTPases · 2020Review
- Microtubule disruption changes endothelial cell mechanics and adhesion.Scientific reports · 2019Article
- Regulation of nuclear architecture, mechanics, and nucleocytoplasmic shuttling of epigenetic factors by cell geometric constraints.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Microtubule-Based Control of Motor-Clutch System Mechanics in Glioma Cell Migration.Cell reports · 2018Article
- Curvotaxis directs cell migration through cell-scale curvature landscapes.Nature communications · 2018Article
- Mechanosensing drives acuity ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Single and collective cell migration: the mechanics of adhesions.Molecular biology of the cell · 2017Article
- O-GlcNAcylation promotes migration and invasion in human ovarian cancer cells via the RhoA/ROCK/MLC pathway.Molecular medicine reports · 2017Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Microtubules have been proposed to function as rigid struts which oppose cellular contraction. Consistent with this hypothesis, microtubule disruption strengthens the contractile force exerted by many cell types. We have investigated alternative explanation for the mechanical effects of microtubule disruption: that microtubules modulate the mechanochemical activity of myosin by influencing phosphorylation of the myosin regulatory light chain (LC20). We measured the force produced by a population of fibroblasts within a collagen lattice attached to an isometric force transducer. Treatment of cells with nocodazole, an inhibitor of microtubule polymerization, stimulated an isometric contraction that reached its peak level within 30 min and was typically 30-45% of the force increase following maximal stimulation with 30% fetal bovine serum. The contraction following nocodazole treatment was associated with a 2- to 4-fold increase in LC20 phosphorylation. The increases in both force and LC20 phosphorylation, after addition of nocodazole, could be blocked or reversed by stabilizing the microtubules with paclitaxel (former generic name, taxol). Increasing force and LC20 phosphorylation by pretreatment with fetal bovine serum decreased the subsequent additional contraction upon microtubule disruption, a finding that appears inconsistent with a load-shifting mechanism. Our results suggest that phosphorylation of LC20 is a common mechanism for the contractions stimulated both by microtubule poisons and receptor-mediated agonists. The modulation of myosin activity by alterations in microtubule assembly may coordinate the physiological functions of these cytoskeletal components.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.