ArticleProceedings of the National Academy of Sciences of the United States of America1995
Subunit interactions in coordination of Ni2+ in cyclic nucleotide-gated channels.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 107 citations in OpenAlex.
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- Regulation of calcium homeostasis in the outer segments of rod and cone photoreceptors.Progress in retinal and eye research · 2018Review
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- Regulation of CNGA1 Channel Gating by Interactions with the Membrane.The Journal of biological chemistry · 2016Article
- Effect of knocking down the insulin receptor on mouse rod responses.Scientific reports · 2015Article
- Coarse architecture of the transient receptor potential vanilloid 1 (TRPV1) ion channel determined by fluorescence resonance energy transfer.The Journal of biological chemistry · 2013Article
- A secondary structural transition in the C-helix promotes gating of cyclic nucleotide-regulated ion channels.The Journal of biological chemistry · 2013Article
- Insulin receptor regulates photoreceptor CNG channel activity.American journal of physiology. Endocrinology and metabolism · 2012Article
- Gating in CNGA1 channels.Pflugers Archiv : European journal of physiology · 2010Review
- Preferential KAT1-KAT2 heteromerization determines inward K+ current properties in Arabidopsis guard cells.The Journal of biological chemistry · 2010Article
- A comparison of electrophysiological properties of the CNGA1, CNGA1tandem and CNGA1cys-free channels.European biophysics journal : EBJ · 2008Article
- An extracellular Cu2+ binding site in the voltage sensor of BK and Shaker potassium channels.The Journal of general physiology · 2008Article
- Trace metals in the brain: allosteric modulators of ligand-gated receptor channels, the case of ATP-gated P2X receptors.European biophysics journal : EBJ · 2008Review
- The structure of the prokaryotic cyclic nucleotide-modulated potassium channel MloK1 at 16 A resolution.Structure (London, England : 1993) · 2007Article
- Stoichiometry studies reveal functional properties of KDC1 in plant shaker potassium channels.Biophysical journal · 2006Article
- State-independent block of BK channels by an intracellular quaternary ammonium.The Journal of general physiology · 2006Article
- Locking CNGA1 channels in the open and closed state.Biophysical journal · 2006Article
- The pharmacology of cyclic nucleotide-gated channels: emerging from the darkness.Current pharmaceutical design · 2006Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Cyclic nucleotide-gated (CNG) channels present a unique model for studying the molecular mechanisms of channel gating. We have studied the mechanism of potentiation of expressed rod CNG channels by Ni2+ as a first step toward understanding the channel gating process. Here we report that coordination of Ni2+ between histidine residues (H420) on adjacent channel subunits occurs when the channels are open. Mutation of H420 to lysine completely eliminated the potentiation by Ni2+ but did not markedly alter the apparent cGMP affinity of the channel, indicating that the introduction of positive charge at the Ni(2+)-binding site was not sufficient to produce potentiation. Deletion or mutation of most of the other histidines present in the channel did not diminish potentiation by Ni2+. We studied the role of subunit interactions in Ni2+ potentiation by generating heteromultimeric channels using tandem dimers of the rod CNG channel sequence. Injection of single heterodimers in which one subunit contained H420 and the other did not (wt/H420Q or H420Q/wt) resulted in channels that were not potentiated by Ni2+. However, coinjection of both heterodimers into Xenopus oocytes resulted in channels that exhibited potentiation. The H420 residues probably occurred predominantly in nonadjacent subunits when each heterodimer was injected individually, but, when the two heterodimers were coinjected, the H420 residues could occur in adjacent subunits as well. These results suggest that the mechanism of Ni2+ potentiation involves intersubunit coordination of Ni2+ by H420. Based on the preferential binding of Ni2+ to open channels, we suggest that alignment of H420 residues of neighboring subunits into the Ni(2+)-coordinating position may be associated with channel opening.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.