ArticleParasitology research1995
Immunology and morphology studies on the proliferation of in vitro cultivated Echinococcus multilocularis metacestodes.
Article in Parasitology research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 97 citations in OpenAlex.
- Monoclonal antibody-based localization of major diagnostic antigens in metacestode tissue, excretory/secretory products, and extracellular vesicles ofFrontiers in cellular and infection microbiology · 2023Article
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- Echinococcus granulosus Protoscolex DM9 Protein Shows High Potential for Serodiagnosis of Alveolar Echinococcosis.The Korean journal of parasitology · 2022Article
- Metformin improves the therapeutic efficacy of low-dose albendazole against experimental alveolar echinococcosis.Parasitology · 2022Article
- Article
- The importance of being parasiticidal… an update on drug development for the treatment of alveolar echinococcosis.Food and waterborne parasitology · 2019Article
- Calmodulin-specific small interfering RNA induces consistent expression suppression and morphological changes in Echinococcus granulosus.Scientific reports · 2019Article
- Impact of affected lymph nodes on long-term outcome after surgical therapy of alveolar echinococcosis.Langenbeck's archives of surgery · 2018Article
- Article
- In vitro culture of Echinococcus multilocularis producing protoscoleces and mouse infection with the cultured vesicles.Parasites & vectors · 2016Article
- An Echinococcus multilocularis Antigen B3 Proteoform That Shows Specific Antibody Responses to Active-Stage Alveolar Echinococcosis.Journal of clinical microbiology · 2015Article
- Prevention and Immunotherapy of Secondary Murine Alveolar Echinococcosis Employing Recombinant EmP29 Antigen.PLoS neglected tropical diseases · 2015Article
- In vitro efficacy of triclabendazole and clorsulon against the larval stage of Echinococcus multilocularis.Parasitology research · 2013Article
- Galα1-4Galβ1-3GalNAc is the dominant epitope of Em2 antigen, the mucin-type glycoprotein from Echinococcus multilocularis.Parasitology research · 2012Article
- In vitro efficacy of dicationic compounds and mefloquine enantiomers against Echinococcus multilocularis metacestodes.Antimicrobial agents and chemotherapy · 2011Article
- Morphological and biological characterization of cell line developed from bovine Echinococcus granulosus.In vitro cellular & developmental biology. Animal · 2010Article
- The role of evolutionarily conserved signalling systems in Echinococcus multilocularis development and host-parasite interaction.Medical microbiology and immunology · 2010Review
- Effects of in vitro exposure of Echinococcus multilocularis metacestodes to cytostatic drugs on in vivo growth and proliferation of the parasite.Parasitology research · 2010Article
- Echinococcus multilocularis metacestode metabolites contain a cysteine protease that digests eotaxin, a CC pro-inflammatory chemokine.Parasitology research · 2009Article
- In vitro and in vivo treatments of echinococcus protoscoleces and metacestodes with artemisinin and artemisinin derivatives.Antimicrobial agents and chemotherapy · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The larval stage of Echinococcus multilocularis causes alveolar echinococcosis (AE) in various mammals, including humans. Traditionally metacestodes are maintained in the laboratory by serial transplantation passages into susceptible animals such as mice or gerbils. However, in animal models it has always been difficult to draw definite conclusions about the factors modulating metacestode differentiation, and investigations on gene expression and respective regulation have been hampered by the complexicity of the host-parasite interplay. This paper describes the maintenance and proliferation of E. multilocularis metacestodes as well as the formation of protoscolices in a chemically defined medium devoid of host influence. The interactive role of a heterologous human cell line (CACO2) in the in vitro development of metacestodes was also assessed. The morphology and ultrastructure of in vitro-generated metacestodes was studied using scanning (SEM) and transmission electron microscopy (TEM). Different cultivation procedures were analyzed in terms of expression of B- and T-cell epitopes and of the relevant laminated layer-antigen Em2; the exact localization of this antigen was further demonstrated by immunogold electron microscopy.
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