ArticlePflugers Archiv : European journal of physiology1995
Protein kinase C consensus sites and the regulation of renal Na/Pi-cotransport (NaPi-2) expressed in XENOPUS laevis oocytes.
Article in Pflugers Archiv : European journal of physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 53 citations in OpenAlex.
- Review
- Effects of single family room architecture on parent-infant closeness and family centered care in neonatal environments-a single-center pre-post study.Journal of perinatology : official journal of the California Perinatal Association · 2021Article
- Role of the putative PKC phosphorylation sites of the type IIc sodium-dependent phosphate transporter in parathyroid hormone regulation.Clinical and experimental nephrology · 2019Article
- Regulation of the human Na+-dependent glucose cotransporter hSGLT2.American journal of physiology. Cell physiology · 2012Article
- Functional interaction between CFTR and the sodium-phosphate co-transport type 2a in Xenopus laevis oocytes.PloS one · 2012Article
- Regulation of organic cation transport.Pflugers Archiv : European journal of physiology · 2005Review
- A dibasic motif involved in parathyroid hormone-induced down-regulation of the type IIa NaPi cotransporter.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Direct and reversed amino acid sequence pattern analysis: structural reasons for activity of reversed sequence sites and results of kinase site mutagenesis.The Biochemical journal · 2000Article
- Protein kinase C activators induce membrane retrieval of type II Na+-phosphate cotransporters expressed in Xenopus oocytes.The Journal of physiology · 1999Article
- Effects of dietary Pi on the renal Na+-dependent Pi transporter NaPi-2 in thyroparathyroidectomized rats.The Biochemical journal · 1998Article
- The renal type II Na+/phosphate cotransporter.Journal of bioenergetics and biomembranes · 1998Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Renal brush border membrane sodium/phosphate (Na/Pi)-cotransport activity is inhibited by hormonal mechanisms involving activation of protein kinases A and C. The recently cloned rat renal Na/Pi-cotransporter (NaPi-2) contains several protein kinase C but no protein kinase A consensus sites [17, 20]. In the present study we have expressed wild type and polymutant (protein kinase C consensus sites removed) NaPi-2-transporters in Xenopus laevis oocytes. The expression of transport function as well as the basic transport properties were unaffected by the removal of the consensus sites. Pharmacological activation of protein kinase C with phorbol 12,13-didecanoate (PDD) led to a time-dependent inhibition of expressed wild type Na/Pi-cotransport function; simultaneous exposure to staurosporine (0.3) prevented the PDD induced (50 nM) inhibition. The kinase-C-mediated inhibition was not prevented by the removal of the protein kinase C consensus sites. Pharmacological activation of protein kinase A (dibutyryl adenosine 3':5':cyclic monophosphate (cAMP)/forskolin) had no effect on wild type NaPi-2-induced oocyte Na/Pi-cotransport. It is concluded that the protein-kinase-C-mediated regulation of expressed Na/Pi-cotransport does not involve the predicted consensus sites. The involvement of "cryptic" phosphorylation sites and/or of a phosphorylated "regulatory" protein is discussed.
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