ArticlePflugers Archiv : European journal of physiology1995
Polarized ion transport during migration of transformed Madin-Darby canine kidney cells.
Article in Pflugers Archiv : European journal of physiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 63 citations in OpenAlex.
- pH-regulated single cell migration.Pflugers Archiv : European journal of physiology · 2024Review
- Ion Channels in Cancer: Orchestrators of Electrical Signaling and Cellular Crosstalk.Reviews of physiology, biochemistry and pharmacology · 2022Review
- Cell volume regulation in cancer cell migration driven by osmotic water flow.Cancer science · 2019Review
- The Volume-Regulated Anion Channel LRRC8/VRAC Is Dispensable for Cell Proliferation and Migration.International journal of molecular sciences · 2019Article
- Planarian regeneration as a model of anatomical homeostasis: Recent progress in biophysical and computational approaches.Seminars in cell & developmental biology · 2019Review
- EAG2 potassium channel with evolutionarily conserved function as a brain tumor target.Nature neuroscience · 2015Article
- Article
- Molecular bioelectricity: how endogenous voltage potentials control cell behavior and instruct pattern regulation in vivo.Molecular biology of the cell · 2014Article
- TRP channels and STIM/ORAI proteins: sensors and effectors of cancer and stroma cell migration.British journal of pharmacology · 2014Review
- cAMP-PKA inhibition of SK3 channel reduced both Ca2+ entry and cancer cell migration by regulation of SK3-Orai1 complex.Pflugers Archiv : European journal of physiology · 2014Article
- Evidence of K+ channel function in epithelial cell migration, proliferation, and repair.American journal of physiology. Cell physiology · 2014Review
- Intermediate conductance Ca2+-activated K+ channels modulate human placental trophoblast syncytialization.PloS one · 2014Article
- The role of TMEM16A (ANO1) and TMEM16F (ANO6) in cell migration.Pflugers Archiv : European journal of physiology · 2013Article
- Endogenous voltage gradients as mediators of cell-cell communication: strategies for investigating bioelectrical signals during pattern formation.Cell and tissue research · 2013Review
- Regulation of cell behavior and tissue patterning by bioelectrical signals: challenges and opportunities for biomedical engineering.Annual review of biomedical engineering · 2012Review
- Regulation of brain tumor dispersal by NKCC1 through a novel role in focal adhesion regulation.PLoS biology · 2012Article
- The inhibition of KCa3.1 channels activity reduces cell motility in glioblastoma derived cancer stem cells.PloS one · 2012Article
- Functional KCa3.1 K+ channels are required for human fibrocyte migration.The Journal of allergy and clinical immunology · 2011Article
- Ion channels and transporters [corrected] in cancer. 2. Ion channels and the control of cancer cell migration.American journal of physiology. Cell physiology · 2011Review
- Lysophosphatidylcholine- and MCP-1-induced chemotaxis of monocytes requires potassium channel activity.Pflugers Archiv : European journal of physiology · 2009Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epithelial cells lose their usual polarization during carcinogenesis. Although most malignant tumours are of epithelial origin little is known about ion channels in carcinoma cells. Previously, we observed that migration of transformed Madin-Darby canine kidney (MDCK-F) cells depended on oscillating K+ channel activity. In the present study we examined whether periodic K+ channel activity may cause changes of cell volume, and whether K+ channel activity is distributed in a uniform way in MDCK-F cells. After determining the average volume of MDCK-F cells (2013+/-270 microm3; n=8) by means of atomic force microscopy we deduced volume changes by calculating the K+ efflux during bursts of K+ channel activity. Therefore, we measured the membrane conductance of MDCK-F cells which periodically rose by 22.3+/-2.5 nS from a resting level of 6.5+/-1.4 nS (n=12), and we measured the membrane potential which hyperpolarized in parallel from -35.4+/-1.2 mV to -71.6+/-1.8 mV (n=11). The distribution of K+ channel activity was assessed by locally superfusing the front or rear end of migrating MDCK-F cells with the K+ channel blocker charybdotoxin (CTX). Only exposure of the rear end to CTX inhibited migration providing evidence for "horizontal" polarization of K+ channel activity in transformed MDCK-F cells. This is in contrast to the "vertical" polarization in parent MDCK cells. We propose that the asymmetrical distribution of K+ channel activity is a prerequisite for migration of MDCK-F cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.