ArticleMolecular & general genetics : MGG1995
Heat shock and light activation of a Chlamydomonas HSP70 gene are mediated by independent regulatory pathways.
Article in Molecular & general genetics : MGG, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 62 citations in OpenAlex.
- UV Radiation and Visible Light Induce hsp70 Gene Expression in the Antarctic Psychrophilic Ciliate Euplotes focardii.Microbial ecology · 2015Article
- Roles of Hsp70s in Stress Responses of Microorganisms, Plants, and Animals.BioMed research international · 2015Review
- Purple chromoprotein gene serves as a new selection marker for transgenesis of the microalga Nannochloropsis oculata.PloS one · 2015Article
- Expression of the high light-inducible Dunaliella LIP promoter in Chlamydomonas reinhardtii.Planta · 2013Article
- Heat shock factor 1 counteracts epigenetic silencing of nuclear transgenes in Chlamydomonas reinhardtii.Nucleic acids research · 2013Article
- Leaf variegation in the rice zebra2 mutant is caused by photoperiodic accumulation of tetra-cis-lycopene and singlet oxygen.Molecules and cells · 2012Article
- Chlorophyll-deficient mutants of Chlamydomonas reinhardtii that accumulate magnesium protoporphyrin IX.Plant molecular biology · 2010Article
- Isolation and characterization of a cDNA encoding a heat shock protein 70 from a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta).Ecotoxicology (London, England) · 2010Article
- Function and regulation of the glutathione peroxidase homologous gene GPXH/GPX5 in Chlamydomonas reinhardtii.Plant molecular biology · 2009Article
- Heme, a plastid-derived regulator of nuclear gene expression in Chlamydomonas.The Plant cell · 2008Article
- The VARL gene family and the evolutionary origins of the master cell-type regulatory gene, regA, in Volvox carteri.Journal of molecular evolution · 2007Article
- In vivo visualization of Mg-protoporphyrin IX, a coordinator of photosynthetic gene expression in the nucleus and the chloroplast.The Plant cell · 2007Article
- Orthologs and paralogs of regA, a master cell-type regulatory gene in Volvox carteri.Current genetics · 2006Article
- Identification of a plastid response element that acts as an enhancer within the Chlamydomonas HSP70A promoter.Nucleic acids research · 2006Article
- Analysis of chromatin structure in the control regions of the chlamydomonas HSP70A and RBCS2 genes.Plant molecular biology · 2005Article
- Thylakoid targeting of Tat passenger proteins shows no delta pH dependence in vivo.The EMBO journal · 2003Article
- The glutathione peroxidase homologous gene from Chlamydomonas reinhardtii is transcriptionally up-regulated by singlet oxygen.Plant molecular biology · 2001Article
- Two enhancers and one silencer located in the introns of regA control somatic cell differentiation in Volvox carteri.Genes & development · 2001Article
- Evidence for non-circadian light/dark-regulated expression of Hsp70s in spinach leaves.Plant physiology · 2001Article
- Identification of short promoter regions involved in the transcriptional expression of the nitrate reductase gene in Chlamydomonas reinhardtii.Plant molecular biology · 2001Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Induction of HSP70 heat shock genes by light has been demonstrated in Chlamydomonas. Our aim was to establish whether this induction by light is mediated by the heat stress sensing pathway or by an independent signal chain. Inhibitors of cytoplasmic protein synthesis revealed an initial difference. Cycloheximide and other inhibitors of protein synthesis prevented HSP70A induction upon illumination but not during heat stress. Analysis of HSP70A induction in cells that had differentiated into gametes revealed a second difference. While heat shock resulted in elevated HSP70A mRNA levels, light was no longer able to serve as an inducer in gametes. To identify the regulatory sequences that mediate the response of the HSP70A gene to either heat stress or light we introduced a series of progressive 5' truncations into its promoter sequence. Analyses of the levels of mRNA transcribed from these deletion constructs showed that in most of them the responses to heat shock and light were similar, suggesting that light induction is mediated by a light-activated heat shock factor. However, we show that the HSP70A promoter also contains cis-acting sequences involved in light induction that do not participate in induction by heat stress. Together, these results provide evidence for a regulation of HSP70A gene expression by light through a heat shock-independent signal pathway.
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