ArticleMolecular & general genetics : MGG1995
Arabidopsis thaliana tryptophan synthase alpha: gene cloning, expression, and subunit interaction.
Article in Molecular & general genetics : MGG, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 52 citations in OpenAlex.
- Cloning and characterization of indole synthase (INS) and a putative tryptophan synthase α-subunit (TSA) genes from Polygonum tinctorium.Plant cell reports · 2016Article
- Something Old, Something New: Conserved Enzymes and the Evolution of Novelty in Plant Specialized Metabolism.Plant physiology · 2015Review
- Tryptophan-independent auxin biosynthesis contributes to early embryogenesis in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The Biosynthetic Pathways for Shikimate and Aromatic Amino Acids in Arabidopsis thaliana.The arabidopsis book · 2010Article
- Comparative transcriptomic characterization of aluminum, sodium chloride, cadmium and copper rhizotoxicities in Arabidopsis thaliana.BMC plant biology · 2009Article
- Mutation of a rice gene encoding a phenylalanine biosynthetic enzyme results in accumulation of phenylalanine and tryptophan.The Plant cell · 2008Article
- Characterisation of the tryptophan synthase alpha subunit in maize.BMC plant biology · 2008Article
- Molecular characterization of two anthranilate synthase alpha subunit genes in Camptotheca acuminata.Planta · 2005Article
- Jasmonate-dependent induction of indole glucosinolates in Arabidopsis by culture filtrates of the nonspecific pathogen Erwinia carotovora.Plant physiology · 2001Article
- An herbivore elicitor activates the gene for indole emission in maize.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Intron-mediated enhancement of gene expression independent of unique intron sequences and splicing.Plant physiology · 2000Article
- Biosynthesis of camalexin from tryptophan pathway intermediates in cell-suspension cultures of Arabidopsis.Plant physiology · 1998Article
- Identification of the gene encoding the tryptophan synthase beta-subunit from Chlamydomonas reinhardtii.Plant physiology · 1998Article
- A deletion in an indole synthase gene is responsible for the DIMBOA-deficient phenotype of bxbx maize.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Coordinate regulation of the tryptophan biosynthetic pathway and indolic phytoalexin accumulation in Arabidopsis.The Plant cell · 1996Article
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3 authors at 1 institution in 1 country.
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Abstract
The tryptophan synthase alpha subunit catalyzes the conversion of indole-3-glycerolphosphate to indole, the penultimate reaction in the biosynthesis of the essential amino acid tryptophan. A cDNA encoding Arabidopsis thaliana tryptophan synthase alpha(TSA1) was isolated by complementation of an Escherichia coli delta trpA mutation and by polymerase chain reaction amplification from a cDNA library using degenerate primers. A TSA1 genomic clone was also isolated and 5 kb of the DNA sequence determined. A single sequence in the Arabidopsis genome with homology to the TSA1 cDNA was detected by high-stringency genomic Southern blot hybridization. In contrast under hybridization conditions of reduced stringency, one or two additional homologous sequences were observed. A 1.4 kb transcript was detected in wild-type RNA with the TSA1 cDNA as a probe. Several lines of evidence, including immunoaffinity chromatography, suggest that the active A. thaliana tryptophan synthase enzyme consists of a heterosubunit complex, presumably analogous to the prokaryotic alpha 2 beta 2 complex. Immunoblot analysis indicated that the plant alpha and beta subunits are present throughout development.
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