ArticleHistochemistry1980
Histochemical demonstration of thiols and disulfides by the fluorescent labeling agent, monobromobimane: an application to the hypothalamo-neurohypophysial system.
Article in Histochemistry, 1980. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed.
- The chronological evolution of small organic molecular fluorescent probes for thiols.Chemical science · 2020Review
- Glutathione and gamma-glutamyl transpeptidase are differentially distributed in the olfactory mucosa of rats.Cell and tissue research · 1992Article
- Comparative investigations of the morphology and chemical composition of the eggshells of Acanthocephala. I. Macracanthorhynchus hirudinaceus (Archiacanthocephala).Parasitology research · 1991Article
- Bromobimanes--fluorescent labeling agents for histochemical detection of sulfur containing neuropeptides in semithin sections.Histochemistry · 1987Article
- Histochemistry of sulfhydryls in acute myocardial infarction.Virchows Archiv. A, Pathological anatomy and histopathology · 1985Article
- Bimane-labelled pepstatin, a fluorescent probe for the subcellular location of cathepsin D.The Biochemical journal · 1981Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of the new fluorescent labeling agent monobromobimane for the histological visualization of thiols, and, after reduction, of disulfides, is described. Fluorescent labeling of the disulfide-rich neurosecretory proteins in the hypothalamo-neurohypophysial system of rat and frog is shown. The reaction of the non-fluorescent reagent with thiols results in highly fluorescent materials which resist fading. The labeling procedure is very simple, rapid, and is carried out in one step. It is non-destructive and can allow for biochemical analyses of the labeled tissue.
Indexed as
Identifiers
7462005What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.