Evidence map›Paper›PMID 7433088›Full record

ArticleNucleic acids research1980

Identification of recombinant phages containing sequences from different rat myosin heavy chain genes.

U Nudel, D Katcoff, Y Carmon, D Zevin-Sonkin, Z Levi, Y Shaul, M Shani, D Yaffe

Open access · bronzeAbstract readComparative Study
In one paragraph

Article in Nucleic acids research, 1980. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 54 citations in OpenAlex.

  1. Farewell to Professor David Yaffe - A pillar of the myogenesis field.European journal of translational myology · 2020
    Article
  2. Article
  3. Slow myosin in developing rat skeletal muscle.The Journal of cell biology · 1987
    Article
  4. Article
  5. Immunological identification of the genes encoding the four myosin heavy chain isoforms of Caenorhabditis elegans.Proceedings of the National Academy of Sciences of the United States of America · 1986
    Article
  6. Mutations of the Drosophila myosin heavy-chain gene: effects on transcription, myosin accumulation, and muscle function.Proceedings of the National Academy of Sciences of the United States of America · 1986
    Article
  7. Article
  8. Article
  9. Article
  10. Cardiac alpha- and beta-myosin heavy chain genes are organized in tandem.Proceedings of the National Academy of Sciences of the United States of America · 1984
    Article
  11. Article
  12. Article
  13. Sarcomeric myosin heavy chain is coded by a highly conserved multigene family.Proceedings of the National Academy of Sciences of the United States of America · 1982
    Article
  14. Isolation and characterization of rat skeletal muscle and cytoplasmic actin genes.Proceedings of the National Academy of Sciences of the United States of America · 1982
    Article
  15. Article
  16. Molecular cloning of two fast myosin heavy chain cDNAs from chicken embryo skeletal muscle.Proceedings of the National Academy of Sciences of the United States of America · 1981
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

U Nudel
D Katcoff
Y Carmon
D Zevin-Sonkin
Z Levi
Y Shaul
M Shani
D Yaffe
Weizmann Institute of Science · IL

Funding

CONTROL OF DIFFERENTIATION IN ESTABLISHED CELL LINESR01GM022767 · NIGMS · WEIZMANN INSTITUTE OF SCIENCE · PI YAFFE, DAVID · 1985 to 1986
–
NIGMS NIH HHS R01-GM-22767
6 · The paper itself

Abstract

The construction and identification of a recombinant plasmid containing a cDNA insert which hybridizes specifically to myosin heavy chain mRNA is described. The plasmid was used as a probe to screen a rat genomic library for recombinant phages containing myosin heavy chain sequences. Six clones with approximately 15 k bp inserts each were isolated. Digestion with several restriction enzymes and hybridization of the fractionated DNA with the plasmid probe showed that the clones contained 3 different DNA inserts. Electron microscopy of a heteroduplex made by hybridization of DNA from two clones confirmed that the inserts originated in different genes. Hybridization of size-fractionated ECOR1 digested rat spleen DNA with the cloned probe suggested the existence of at least 5 myosin heavy chain genes.

Indexed as

DNA, RecombinantGene AmplificationGenesAnimalsCloning, MolecularColiphagesMyosinsNucleic Acid HeteroduplexesNucleic Acid HybridizationPlasmidsRatsRNA, MessengerDNA, RecombinantMyosinsNucleic Acid HeteroduplexesRNA, Messenger

Identifiers

PMID7433088
PMCPMC324068
OpenAlexW2013310702

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.