ArticleThe Journal of cell biology1981
Endocytosis by human platelets: metabolic and freeze-fracture studies.
Article in The Journal of cell biology, 1981. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 77 citations in OpenAlex.
- New insights of platelet endocytosis and its implication for platelet function.Frontiers in cardiovascular medicine · 2023Review
- Platelet-derived extracellular vesicles infiltrate and modify the bone marrow during inflammation.Blood advances · 2020Article
- microRNAs as promising biomarkers of platelet activity in antiplatelet therapy monitoring.International journal of molecular sciences · 2020Review
- Article
- The ins and outs of endocytic trafficking in platelet functions.Current opinion in hematology · 2017Review
- Platelet secretion in inflammatory and infectious diseases.Platelets · 2017Review
- Article
- Article
- Isolation and characterization of platelet-derived extracellular vesicles.Journal of extracellular vesicles · 2014Article
- Platelet alpha-granules: basic biology and clinical correlates.Blood reviews · 2009Review
- Storage lesion of human platelets as revealed by ultrathin sections and freeze-fracture replicas.Cell and tissue research · 1994Article
- Plasma membrane GPIIb/IIIa. Evidence for a cycling receptor pool.The American journal of pathology · 1990Article
- Distribution of glycoconjugates on the plasma membrane and on membranes of the open-canalicular system in human platelets. A cytochemical study.Histochemistry · 1989Article
- Antigenic differences between human platelets and megakaryocytes.The American journal of pathology · 1987Article
- Incorporation of a circulating protein into megakaryocyte and platelet granules.Proceedings of the National Academy of Sciences of the United States of America · 1987Article
- Loss of intercalated membrane particles by treatment with phorbols.Proceedings of the National Academy of Sciences of the United States of America · 1986Article
- Localization of internal pools of membrane glycoproteins involved in platelet adhesive responses.The American journal of pathology · 1986Article
- Freeze-fracture cytochemistry of wheat germ agglutinin and concanavalin A receptors on the plasma membrane of normal, Bernard-Soulier, and thrombasthenic platelets.The American journal of pathology · 1986Article
- Rearrangement of the open-canalicular system of the human blood platelet after incorporation of surface-bound ligands. A high-voltage electron-microscopic study.Cell and tissue research · 1986Article
- Thrombocytopoiesis--analysis by membrane tracer and freeze-fracture studies on fresh human and cultured mouse megakaryocytes.The Journal of cell biology · 1984Article
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Authors and funding
1 author.
Funding
Abstract
The mechanism by which platelets endocytose or release particulate or soluble substances is poorly understood. Engulfed materials enter the open canalicular system (OCS) by a process akin to phagocytosis, but fusion of platelet granules with the OCS is rarely observed. Secretion of granule contents, a concomitant of the "release reaction" which occurs during platelet aggregation, does not take place by extrusion at the surface membrane as is true for other secretory cells. Some substances may be secreted without obvious granule loss. To examine whether structural properties of the platelet membrane could account for this unusual behavior, thin section and freeze-fracture analyses were performed on platelets which had undergone endocytosis under a variety of experimental conditions. After freeze-cleavage, most of the intramembranous particles (IMP) remain associated with the outer leaflet of the platelet plasma membrane. The sites where the OCS reaches the surface membrane are marked by pits on the cytoplasmic leaflet (P face) and by complementary protrusions on the outer leaflet (E face) of the membrane. Endocytosis of small particles and solutes takes place via these structures. This process is not energy dependent but arrested at 4 degrees C. Distension of the OCS does not appear to affect the size or number of the pits. On the other hand, large particles are taken up by membrane invagination without redistribution of IMP's and independent of the pits. This process is sensitive to metabolic inhibition. Thus, the studies have demonstrated the existence of two different pathways for platelet endocytosis which are postulated to be also involved in secretion. The selective release of substances contained in different granules may be related to the "inside-out" structure of the plasma and OCS membranes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.