ArticleBiophysical journal1981
Fluorescence photobleaching recovery in solutions of labeled actin.
Article in Biophysical journal, 1981. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 61 citations in OpenAlex.
- Improved model of fluorescence recovery expands the application of multiphoton fluorescence recovery after photobleaching in vivo.Biophysical journal · 2009Article
- Chromophore-assisted laser inactivation in cell biology.Trends in cell biology · 2008Review
- Diffusion rate limitations in actin-based propulsion of hard and deformable particles.Biophysical journal · 2006Article
- Growing an actin gel on spherical surfaces.Biophysical journal · 2000Article
- An analysis of actin delivery in the acrosomal process of thyone.Biophysical journal · 1999Article
- Fluorescence photobleaching with spatial Fourier analysis: measurement of diffusion in light-scattering media.Biophysical journal · 1993Article
- Flow rate measurements in isolated perfused kidney tubules by fluorescence photobleaching recovery.Biophysical journal · 1991Article
- A continuum model of protrusion of pseudopod in leukocytes.Biophysical journal · 1988Article
- Analysis of rhodamine and fluorescein-labeled F-actin diffusion in vitro by fluorescence photobleaching recovery.Biophysical journal · 1988Article
- Actin assembly by lithium ions.Biophysical journal · 1988Article
- Molecular aggregation characterized by high order autocorrelation in fluorescence correlation spectroscopy.Biophysical journal · 1987Article
- Acanthamoeba profilin binding to fluorescein-labeled actins.Biophysical journal · 1987Article
- Assembly of actin filaments studied by laser light scattering and fluorescence photobleaching recovery.Biophysical journal · 1986Article
- Behavior of a fluorescent analogue of calmodulin in living 3T3 cells.The Journal of cell biology · 1985Article
- Time-resolved X-ray scattering study of actin polymerization from profilactin.European biophysics journal : EBJ · 1985Article
- Separation of translational and rotational contributions in solution studies using fluorescence photobleaching recovery.Biophysical journal · 1984Article
- Assembly properties of fluorescein-labeled tubulin in vitro before and after fluorescence bleaching.The Journal of cell biology · 1984Article
- Spindle microtubule dynamics in sea urchin embryos: analysis using a fluorescein-labeled tubulin and measurements of fluorescence redistribution after laser photobleaching.The Journal of cell biology · 1984Article
- Detection and characterization of actin monomers, oligomers, and filaments in solution by measurement of fluorescence photobleaching recovery.Biophysical journal · 1984Article
- A localized pattern photobleaching method for the concurrent analysis of rapid and slow diffusion processes.Biophysical journal · 1983Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have demonstrated that the technique of fluorescence photobleaching recovery (FPR) can be used to examine the state of a single component in complex self-assembling macromolecular systems. Polymerization of actin, initiated by addition of salt or Mg+2 to a low-ionic-strength solution of G-actin, has been observed by sequential measurement of FPR with the aid of fluorescein-labeled actin. Solutions of actin which had been labeled using 5-iodoacetamido fluorescein (5-IAF) showed anomalous recovery of fluorescence above the initial value, which indicates a photoinduced increase in local polymerization. No such anomaly was observed with actin that had been labeled with fluorescein isothiocyanate (FITC). The FPR data are directly interpretable in terms of the fraction of labeled protein that is immobilized in the supramolecular assembly and in terms of the average diffusion coefficient of the mobile fraction. Our data are consistent with the "treadmill" model of actin polymerization, in that they show that actin is present under polymerizing conditions either as a high polymer or as monomer or low oligomer. We believe that the FPR technique can be applied to the study of many types of reconstituted motile or cytoskeletal systems in vitro or in vivo.
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