ArticleCell and tissue research1982
Spatial organization and fine structure of the cortical filament layer in normal locomoting Amoeba proteus.
Article in Cell and tissue research, 1982. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 66 citations in OpenAlex.
- Effects of nocodazole and latrunculin B on locomotion of amoeboid cells of Rhizochromulina sp. strain B44 (Heterokontophyta, Dictyochophyceae).Protoplasma · 2025Article
- From Molecules to Amoeboid Movement: A New Way for Understanding the Morphology Through Actin-Binding Proteins.Biomolecules · 2024Article
- Light-sheet microscopy reveals dorsoventral asymmetric membrane dynamics of Amoeba proteus during pressure-driven locomotion.Biology open · 2023Article
- Blebs-Formation, Regulation, Positioning, and Role in Amoeboid Cell Migration.Frontiers in cell and developmental biology · 2022Review
- Rotation of stress fibers as a single wheel in migrating fish keratocytes.Scientific reports · 2018Article
- Periodic traction in migrating large amoeba of Physarum polycephalum.Journal of the Royal Society, Interface · 2015Article
- Cell migration and division in amoeboid-like fission yeast.Biology open · 2014Article
- Characteristics of motive force derived from trajectory analysis of Amoeba proteus.Protoplasma · 2007Article
- Actin dynamics in Amoeba proteus motility.Protoplasma · 2007Article
- Chaotic behavior in the locomotion of Amoeba proteus.Protoplasma · 2001Article
- Studies on microplasmodia of Physarum polycephalum : VI. Functional analysis of a cortical and fibrillar actin system by use of fluorescent-analog cytochemistry.Cell and tissue research · 1987Article
- Dynamics of the cytoskeleton in Amoeba proteus. I. Redistribution of microinjected fluorescein-labeled actin during locomotion, immobilization and phagocytosis.Cell and tissue research · 1983Article
- Mobility of cytoplasmic and membrane-associated actin in living cells.Proceedings of the National Academy of Sciences of the United States of America · 1982Article
- Visualization of actin polymerization and depolymerization cycles during polyamine-induced cytokinesis in living Amoeba proteus.Cell and tissue research · 1981Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fine structural organization of a cortical filament layer in normal locomoting Amoeba proteus was demonstrated using improved fixation and embedding techniques. Best results were obtained after application of PIPES-buffered glutaraldehyde in connection with substances known to prevent the depolymerization of F-actin, followed by careful dehydration and freeze-substitution. The filament layer is continuous along the entire surface; it exhibits a varying thickness depending on the cell polarity, measuring several nm in advancing regions and 0.5-1 micron in retracting ones. Two different types of filaments are responsible for the construction of the layer: randomly distributed thin (actin) filaments forming an unordered meshwork beneath the plasma membrane, and thick (myosin) filaments mostly restricted to the uroid region in close association with F-actin. The cortical filament layer generates the motive force for amoeboid movement by contraction at posterior cell regions and induces a pressure flow that continues between the uroid with a high hydrostatic pressure and advancing pseudopodia with low one. The local destabilization of the cell surface as a precondition for the formation of pseudopodia is enabled by the detachment of the cortical filament layer from the plasma membrane. This results in morphological changes by the active separation of peripheral hyaloplasmic and central granuloplasmic regions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.