ArticleThe Journal of cell biology1980
Mechanism for the selection of nuclear polypeptides in Xenopus oocytes. II. Two-dimensional gel analysis.
Article in The Journal of cell biology, 1980. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 64 citations in OpenAlex.
- Nucleocytoplasmic transport.The Biochemical journal · 1994Review
- Identification of two HSP70-related Xenopus oocyte proteins that are capable of recycling across the nuclear envelope.The Journal of cell biology · 1990Article
- The function of the nuclear envelope in nuclear protein accumulation.The Journal of cell biology · 1988Article
- The nuclear migration signal of Xenopus laevis nucleoplasmin.The EMBO journal · 1987Article
- Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.The Journal of cell biology · 1987Article
- In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins.The Journal of cell biology · 1986Article
- Assembly in vitro of nuclei active in nuclear protein transport: ATP is required for nucleoplasmin accumulation.The EMBO journal · 1986Article
- Movement of a karyophilic protein through the nuclear pores of oocytes.The Journal of cell biology · 1984Article
- Envelope proteins of Semliki Forest virus synthesized in Xenopus oocytes are transported to the cell surface.The EMBO journal · 1984Article
- Evidence for mediated protein uptake by amphibian oocyte nuclei.The Journal of cell biology · 1983Article
- Karyophilic proteins: polypeptides synthesized in vitro accumulate in the nucleus on microinjection into the cytoplasm of amphibian oocytes.Proceedings of the National Academy of Sciences of the United States of America · 1982Article
- The fate of oocyte nuclear proteins during early development ofXenopus laevis.Wilhelm Roux's archives of developmental biology · 1982Article
- Tissue specific nuclear antigens in the germinal vesicle ofXenopus laevis oocytes.Wilhelm Roux's archives of developmental biology · 1981Article
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Authors and funding
2 authors.
Funding
Abstract
The role of the nuclear envelope in controlling intracellular protein exchanges was investigated in vivo, by determining the effect of altering nuclear permeability on (a) the protein composition of the nucleoplasm and (b) the nuclear uptake rates of specific endogenous proteins. The nuclear envelopes were disrupted by puncturing oocytes in the region of the germinal vesicle by use of glass needles. Nuclear proteins were analyzed in punctured and control cells by two-dimensional gel electrophoresis, fluorography, and double-labeling techniques. Over 300 nuclear polypeptides were identified in the fluorographs. Of this number, only approximately 10-15 were found to vary between punctured and control nuclei; furthermore, different polypeptides varied in each experiment. These qualitative studies indicate that specific binding within the nucleoplasm, and not selection by the envelope, is the main factor in maintaining the protein composition of the nucleus. The nuclear uptake rates of five individual polypeptides, ranging in molecular weight from 43,000 to 100,000, were analyzed by use of double-labeling procedures. Only one of the polypeptides (actin) entered the nuclei more rapidly after disruption of the envelope. That the nuclear uptake of certain endogenous proteins is unaffected by puncturing demonstrates that passage across the envelope is not a rate-limiting step in the nucleocytoplasmic exchange of these molecules.
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