ArticleProceedings of the National Academy of Sciences of the United States of America1981
Characterization of antibodies to smooth muscle myosin kinase and their use in localizing myosin kinase in nonmuscle cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1981. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 89 citations in OpenAlex.
- Article
- MLCK regulates Schwann cell cytoskeletal organization, differentiation and myelination.Journal of cell science · 2011Article
- Characterization of tightly associated smooth muscle myosin-myosin light-chain kinase-calmodulin complexes.Journal of molecular biology · 2009Article
- Multipotent properties of myofibroblast cells derived from human placenta.Cell and tissue research · 2008Article
- Increased myosin light chain kinase expression in hypertension: Regulation by serum response factor via an insertion mutation in the promoter.Molecular biology of the cell · 2006Article
- Inhibiting myosin light chain kinase induces apoptosis in vitro and in vivo.Molecular and cellular biology · 2005Article
- Modulation of myosin light-chain phosphorylation by p21-activated kinase 1 in Escherichia coli invasion of human brain microvascular endothelial cells.Infection and immunity · 2003Article
- A fluorescent resonant energy transfer-based biosensor reveals transient and regional myosin light chain kinase activation in lamella and cleavage furrows.The Journal of cell biology · 2002Article
- Localization and activity of myosin light chain kinase isoforms during the cell cycle.The Journal of cell biology · 2000Article
- Distinct roles of ROCK (Rho-kinase) and MLCK in spatial regulation of MLC phosphorylation for assembly of stress fibers and focal adhesions in 3T3 fibroblasts.The Journal of cell biology · 2000Article
- A calcium signaling cascade essential for myosin thick filament assembly in Xenopus myocytes.The Journal of cell biology · 1998Article
- Regulation of cell motility by mitogen-activated protein kinase.The Journal of cell biology · 1997Article
- Fluorescence anisotropy imaging microscopy maps calmodulin binding during cellular contraction and locomotion.The Journal of cell biology · 1993Article
- Structure and expression of a calcium-binding protein gene contained within a calmodulin-regulated protein kinase gene.Molecular and cellular biology · 1992Article
- The role of myosin I and II in cell motility.Cancer metastasis reviews · 1992Review
- Microinjection of the catalytic fragment of myosin light chain kinase into dividing cells: effects on mitosis and cytokinesis.The Journal of cell biology · 1991Article
- Stopped-flow measurement of cytoskeletal contraction: Dictyostelium myosin II is specifically required for contraction of amoeba cytoskeletons.The Journal of cell biology · 1991Article
- An increase or a decrease in myosin II phosphorylation inhibits macrophage motility.The Journal of cell biology · 1991Article
- Use of DNA sequence and mutant analyses and antisense oligodeoxynucleotides to examine the molecular basis of nonmuscle myosin light chain kinase autoinhibition, calmodulin recognition, and activity.The Journal of cell biology · 1990Article
- Protein phosphatase type-1, not type-2A, modulates actin microfilament integrity and myosin light chain phosphorylation in living nonmuscle cells.The Journal of cell biology · 1990Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Antibodies to myosin light chain kinase, purified from turkey gizzard smooth muscle, were developed in rabbits and purified by affinity chromatography on a myosin light chain kinase-Sepharose 4B column. The purified antibodies crossreact with purified smooth muscle myosin light chain kinase but not with a variety of contractile or cytoskeletal proteins. The antibodies inhibit the catalytic activity of smooth muscle myosin light chain kinase and there is an inverse relationship between the kinase activity and the amount of antibody present in an assay. Half-maximal inhibition of myosin kinase activity occurs at an antibody/myosin kinase molar ratio of 10:1. The affinity-purified antibodies to smooth muscle myosin kinase were used to study the location of myosin kinase in a variety of nonmuscle cells. Immunofluorescence studies indicate that myosin light chain kinase is localized on microfilament bundles (stress fibers) in cultured fibroblasts. The stress fiber staining pattern is abolished when the antibodies are incubated with purified smooth muscle myosin light chain kinase prior to staining cells, while the staining pattern is unaffected when the antibodies are incubated with actin, myosin, alpha-actinin, or tropomyosin prior to staining. Moreover, the stress fiber staining pattern is periodic in well-spread gerbil fibroma cells and experiments have demonstrated that myosin light chain kinase appears to have the same periodic distribution as myosin but an antiperiodic distribution relative to alpha-actinin. These data indicate that myosin light chain kinase and its substrate, myosin, are in close proximity and are consistent with the hypothesis that myosin light chain kinase regulates actin-myosin interactions in nonmuscle cells.
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