ArticleJournal of bacteriology1980
Identification of an actin-like protein and of its messenger ribonucleic acid in Saccharomyces cerevisiae.
Article in Journal of bacteriology, 1980. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- The yeast actin cytoskeleton: from cellular function to biochemical mechanism.Microbiology and molecular biology reviews : MMBR · 2006Review
- The Saccharomyces cerevisiae actin-related protein Arp2 is involved in the actin cytoskeleton.The Journal of cell biology · 1996Article
- The rho-GAP encoded by BEM2 regulates cytoskeletal structure in budding yeast.Molecular biology of the cell · 1995Article
- Yeast actin filaments display ATP-dependent sliding movement over surfaces coated with rabbit muscle myosin.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Lamin A, lamin B, and lamin B receptor analogues in yeast.The Journal of cell biology · 1989Article
- Purification of actin from Candida albicans and comparison with the Candida 48,000-Mr protein.Infection and immunity · 1987Article
- Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene.Proceedings of the National Academy of Sciences of the United States of America · 1984Article
- Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae.The Journal of cell biology · 1984Article
- Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.The Journal of cell biology · 1984Article
- Actin from Saccharomyces cerevisiae.Molecular and cellular biology · 1982Article
- Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae.Molecular and cellular biology · 1982Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We have identified a yeast protein that resembles actins from other eucaryotes in its tight binding to pancreatic deoxyribonuclease I, its copolymerizaton with purified muscle actin, its one-dimensional peptide map, and its apparent polymerization into 7-nm filaments. The yeast actin-like protein yielded a single spot on two-dimensional polyacrylamide gel electrophoresis, suggesting that a single protein species was present. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the actin-like protein had an apparent molecular weight of 45,000 compared with 42,000 for muscle actin. In an attempt to identify the messenger ribonucleic acid coding for the actin-like protein, yeast polyadenylic acid-rich ribonucleic acid was translated in wheat germ and reticulocyte cell-free protein-synthesizing systems. The actin-like protein was identified among the translation products of the reticulocyte system by its tight binding to deoxyribonuclease I, its comigration with the in vivo-synthesized actin-like protein during sodium dodecyl sulfate-polyacrylamide gel electrophoresis, an the similarity of its peptide map to that of the in vivo-synthesized protein. A yeast protein synthesized in the wheat-germ system was also found to bind to deoxyribonuclease I and to copolymerize with muscle actin. However, its apparent molecular weight was about 35,000, suggesting that it was a product either of incomplete translation or of proteolytic cleavage of the actin-like protein.
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