ArticleThe Journal of cell biology1980
A permeabilized cell model for studying cytokinesis using mammalian tissue culture cells.
Article in The Journal of cell biology, 1980. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 78 citations in OpenAlex.
- Cytokinesis in Metazoa and Fungi.Cold Spring Harbor perspectives in biology · 2017Review
- Rho-associated kinase-dependent contraction of stress fibres and the organization of focal adhesions.Journal of the Royal Society, Interface · 2011Review
- Isolation and partial purification of the Saccharomyces cerevisiae cytokinetic apparatus.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Role of stress fibers and focal adhesions as a mediator for mechano-signal transduction in endothelial cells in situ.Vascular health and risk management · 2008Review
- Isolation and contraction of the stress fiber.Molecular biology of the cell · 1998Article
- Calcium control of actin-myosin-based contraction in Triton-treated murine bladder tumor cells.Urological research · 1993Article
- Slow calcium waves accompany cytokinesis in medaka fish eggs.The Journal of cell biology · 1991Article
- Stopped-flow measurement of cytoskeletal contraction: Dictyostelium myosin II is specifically required for contraction of amoeba cytoskeletons.The Journal of cell biology · 1991Article
- Role of actin and myosin in the control of paracellular permeability in pig, rat and human vascular endothelium.The Journal of physiology · 1990Article
- Cleavage furrow isolated from newt eggs: contraction, organization of the actin filaments, and protein components of the furrow.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Evidence for a tubulin-containing lipid-protein structural complex in ciliary membranes.The Journal of cell biology · 1985Article
- Incorporation of serine into Paramecium ethanolamine phospholipid and phosphonolipid head groups.The Biochemical journal · 1984Article
- From proto-mitosis to mitosis--an alternative hypothesis on the origin and evolution of the mitotic spindle.Origins of life · 1984Article
- Regulation of contraction and thick filament assembly-disassembly in glycerinated vertebrate smooth muscle cells.The Journal of cell biology · 1983Article
- N-ethylmaleimide-modified subfragment-1 and heavy meromyosin inhibit reactivated contraction in motile models of retinal cones.The Journal of cell biology · 1983Article
- Analysis of the role of microtubules and actin in erythrophore intracellular motility.The Journal of cell biology · 1983Article
- Isolation and characterization of circumferential microfilament bundles from retinal pigmented epithelial cells.The Journal of cell biology · 1982Article
- A functional in vitro model for studies of intracellular motility in digitonin-permeabilized erythrophores.The Journal of cell biology · 1982Article
- Microinjection into an identified axon to study the mechanism of fast axonal transport.Proceedings of the National Academy of Sciences of the United States of America · 1982Article
- Effects of exogenous proteins on cytoplasmic streaming in perfused Chara cells.The Journal of cell biology · 1982Article
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
PtK1 cells lysed late in cell division in a medium containing the nonionic detergent Brij 58 and polyethylene glycol with continue to undergo cleavage after lysis. Maintenance of cleavage after lysis is dependent on the composition of the lysis medium; the pH must be around neutrality, MgATP must be present, and the free Ca++ concentration should be 1 microM for optimal constriction to occur. Cleavage can be stopped and reinitiated by raising and lowering the Ca++ levels in the lysis medium. Cleavage in the permeabilized cell is blocked by addition of phalloidin, cytochalasin B, and N-ethylmaleimide-modified myosin subfragment-1 to the lysis medium. This represents the first cell model system for studying cleavage since the pioneering studies of Hoffman-Berling in 1954.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.