ArticleProceedings of the National Academy of Sciences of the United States of America1982
Mobility of cytoplasmic and membrane-associated actin in living cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1982. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 101 citations in OpenAlex.
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- Evolution of research on cellular motility over five decades.Biophysical reviews · 2018Review
- Targeting tumor multicellular aggregation through IGPR-1 inhibits colon cancer growth and improves chemotherapy.Oncogenesis · 2017Article
- IGPR-1 Is Required for Endothelial Cell-Cell Adhesion and Barrier Function.Journal of molecular biology · 2016Article
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- Virus cell-to-cell transmission.Journal of virology · 2010Review
- Assembly of the murine leukemia virus is directed towards sites of cell-cell contact.PLoS biology · 2009Article
- Visualization of mRNA translation in living cells.The Journal of cell biology · 2006Article
- Simultaneous measurements of actin filament turnover, filament fraction, and monomer diffusion in endothelial cells.Biophysical journal · 1998Article
- Direct in vivo measurement of targeted binding in a human tumor xenograft.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Interpreting photoactivated fluorescence microscopy measurements of steady-state actin dynamics.Biophysical journal · 1995Article
- A mechanistic role for polypeptide hormone receptor lateral mobility in signal transduction.Amino acids · 1995Article
- Diffusivity of myoglobin in intact skeletal muscle cells.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Focusing on unpolymerized actin.The Journal of cell biology · 1993Review
- In vitro models of tail contraction and cytoplasmic streaming in amoeboid cells.The Journal of cell biology · 1993Article
- Determinants of the translational mobility of a small solute in cell cytoplasm.The Journal of cell biology · 1993Article
- Microtubules are stabilized in confluent epithelial cells but not in fibroblasts.The Journal of cell biology · 1990Article
- Mechanics and control of the cytoskeleton in Amoeba proteus.Biophysical journal · 1989Article
- The dynamic distribution of fluorescent analogues of actin and myosin in protrusions at the leading edge of migrating Swiss 3T3 fibroblasts.The Journal of cell biology · 1988Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have combined fluorescent analogue cytochemistry with fluorescence photobleaching recovery to measure the mobility of fluorescently labeled actin and other labeled test proteins microinjected into living amoebae. Bovine serum albumin, ovalbumin, and ribonuclease A have a cytoplasmic mobility, expressed as a diffusion coefficient, that is 1/2 to 1/3 of that observed in aqueous solution; 90% of the actin has a mobility 1/2 to 1/8 of that of G-actin in aqueous solution, and approximately equal to 10% of the actin has a mobility comparable to that of F-actin in aqueous solution. Therefore, no more than 10% of the actin in the cytoplasm of amoebae can exist as static filaments. Microinjection of phalloidin decreases the diffusion coefficient of the mobile component of cytoplasmic actin, and it also increases the low-mobility fraction to 50% but has no effect on the mobility of labeled ovalbumin. By comparing the mobility of actin in different parts of amoebae and by separating cytoplasm from plasmalemma-ectoplasm, we found the low-mobility fraction of actin to be enriched in the tail, along the plasmalemma-ectoplasm, and in contracted cytoplasm.
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