ArticleThe Journal of cell biology1981
A membrane cytoskeleton from Dictyostelium discoideum. I. Identification and partial characterization of an actin-binding activity.
Article in The Journal of cell biology, 1981. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 99 citations in OpenAlex.
- The cell adhesion molecule DdCAD-1 in Dictyostelium is targeted to the cell surface by a nonclassical transport pathway involving contractile vacuoles.The Journal of cell biology · 1997Article
- The integral membrane protein, ponticulin, acts as a monomer in nucleating actin assembly.The Journal of cell biology · 1993Article
- Nitric oxide stimulates the ADP-ribosylation of a 41-kDa cytosolic protein in Dictyostelium discoideum.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Binding of actin to liver cell membranes: the state of membrane-bound actin.The Journal of cell biology · 1991Article
- Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein.The Journal of cell biology · 1990Article
- How actin binds and assembles onto plasma membranes from Dictyostelium discoideum.The Journal of cell biology · 1988Article
- An unusual protein kinase phosphorylates the chemotactic receptor of Dictyostelium discoideum.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- A 60-kDa cytoskeletal protein from Trypanosoma brucei brucei can interact with membranes and with microtubules.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes.The Journal of cell biology · 1987Article
- Using antibodies against Dictyostelium membranes to identify an actin-binding membrane protein.The Journal of cell biology · 1987Article
- Interaction of the cytoskeleton with the plasma membrane.The Journal of membrane biology · 1987Review
- Junctional plasma membrane domains isolated from aggregating Dictyostelium discoideum amebae.Proceedings of the National Academy of Sciences of the United States of America · 1986Article
- Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum.The Journal of cell biology · 1986Article
- Actin at receptor-rich domains of isolated acetylcholine receptor clusters.The Journal of cell biology · 1986Article
- The human erythrocyte membrane skeleton may be an ionic gel. I. Membrane mechanochemical properties.European biophysics journal : EBJ · 1986Article
- Ligand-induced changes in the location of actin, myosin, 95K (alpha-actinin), and 120K protein in amebae of Dictyostelium discoideum.The Journal of cell biology · 1985Article
- cAMP induces a rapid and reversible modification of the chemotactic receptor in Dictyostelium discoideum.The Journal of cell biology · 1985Article
- Isolation of an actin-binding protein from membranes of Dictyostelium discoideum.The Journal of cell biology · 1985Article
- Isolation of a subpopulation of glycoprotein IIb-III from platelet membranes that is bound to membrane actin.The Journal of cell biology · 1985Article
- Interaction of rat liver lysosomal membranes with actin.The Journal of cell biology · 1984Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Dictyostelium discoideum plasma membranes isolated by each of three procedures bind F-actin. The interactions between these membranes and actin are examined by a novel application of falling ball viscometry. Treating the membranes as multivalent actin-binding particles analogous to divalent actin-gelation factors, we observe large increases in viscosity (actin cross-linking) when membranes of depleted actin and myosin are incubated with rabbit skeletal muscle F-actin. Pre-extraction of peripheral membrane proteins with chaotropes or the inclusion of Triton X-100 during the assay does not appreciably diminish this actin cross-linking activity. Lipid vesicles, heat-denatured membranes, proteolyzed membranes, or membranes containing endogenous actin show minimal actin cross-linking activity. Heat-denatured, but not proteolyzed, membranes regain activity when assayed in the presence of Triton X-100. Thus, integral membrane proteins appear to be responsible for some or all of the actin cross-linking activity of D. discoideum membranes. In the absence of MgATP, Triton X-100 extraction of isolated D. discoideum membranes results in a Triton-insoluble residue composed of actin, myosin, and associated membrane proteins. The inclusion of MgATP before and during Triton extraction greatly diminishes the amount of protein in the Triton-insoluble residue without appreciably altering its composition. Our results suggest the existence of a protein complex stabilized by actin and/or myosin (membrane cytoskeleton) associated with the D. discoideum plasma membrane.
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Registered trials
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