ArticleThe Journal of cell biology1982
Isolation and characterization of circumferential microfilament bundles from retinal pigmented epithelial cells.
Article in The Journal of cell biology, 1982. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 92 citations in OpenAlex.
- Rat corneal endothelial cell migration during wound repair on the basement membrane depends more on the PI-3K pathway than the cdc-42 pathway or actin stress fibers.Cell and tissue research · 2020Article
- Apical constriction: themes and variations on a cellular mechanism driving morphogenesis.Development (Cambridge, England) · 2014Review
- Rho-associated kinase-dependent contraction of stress fibres and the organization of focal adhesions.Journal of the Royal Society, Interface · 2011Review
- ZO-1- and ZO-2-dependent integration of myosin-2 to epithelial zonula adherens.Molecular biology of the cell · 2008Article
- Role of stress fibers and focal adhesions as a mediator for mechano-signal transduction in endothelial cells in situ.Vascular health and risk management · 2008Review
- Retinal pigment epithelial acid lipase activity and lipoprotein receptors: effects of dietary omega-3 fatty acids.Transactions of the American Ophthalmological Society · 2002Article
- Rho-kinase--mediated contraction of isolated stress fibers.The Journal of cell biology · 2001Article
- Isolation and contraction of the stress fiber.Molecular biology of the cell · 1998Article
- Localization of capping protein in chicken epithelial cells by immunofluorescence and biochemical fractionation.The Journal of cell biology · 1992Article
- Ultrastructural and immunocytochemical analysis of the circumferential microfilament bundle in avian retinal pigmented epithelial cells in vitro.Cell and tissue research · 1991Article
- Morphological changes in the zonula adhaerens during embryonic development of chick retinal pigment epithelial cells.Cell and tissue research · 1990Article
- A new 400-kD protein from isolated adherens junctions: its localization at the undercoat of adherens junctions and at microfilament bundles such as stress fibers and circumferential bundles.The Journal of cell biology · 1989Article
- Novel redistribution of myosin-containing filaments in cultured keratinocytes identified by a human monoclonal autoantibody.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1989Article
- Isolation of cell-to-cell adherens junctions from rat liver.The Journal of cell biology · 1989Article
- Cytoskeletons of retinal pigment epithelial cells: interspecies differences of expression patterns indicate independence of cell function from the specific complement of cytoskeletal proteins.Cell and tissue research · 1988Article
- The cytoskeletal mechanics of brain morphogenesis. Cell state splitters cause primary neural induction.Cell biophysics · 1987Review
- The cytoskeleton of chick retinal pigment epithelial cells in situ.Cell and tissue research · 1987Article
- Characterization of intermediate filaments and their structural organization during epithelium formation in pigmented epithelial cells of the retina in vitro.Cell and tissue research · 1986Article
- Increase in actin contents and elongation of apical projections in retinal pigmented epithelial cells during development of the chicken eye.The Journal of cell biology · 1985Article
- Components of the cytoskeleton in the retinal pigmented epithelium of the chick.The Journal of cell biology · 1985Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chicken retinal pigmented epithelial cells have circumferential microfilament bundles (CMBs) at the zonula adherens region. We have isolated these CMBs in intact form and characterized them structurally and biochemically. Pigmented epithelia obtained from 11-d-old chick embryos were treated with glycerol and Triton. Then, the epithelia were homogenized by passing them through syringe needles. Many isolated CMBs were found in the homogenate by phase-contrast microscopy. They formed polygons, mostly pentagons and hexagons, or fragments of polygons. Polygons were filled with meshwork structures, i.e. they were polygonal plates. Upon exposure to Mg-ATP, isolated CMBs showed clear and large contraction. The contraction was inhibited by treatment with N-ethylmaleimide-modified myosin subfragment-1. After purification by centrifugation with the density gradient of Percoll, CMBs were analyzed by SDS PAGE. The electrophoretic pattern gave three major components of 200, 55, and 42 kdaltons and several minor components. Electron microscopy showed that the polygons were composed of thick bundles of actin-containing microfilaments, and the meshworks were composed primarily of intermediate filaments.
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Registered trials
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