ArticleCell and tissue research1983
Organ culture of the small intestine of the suckling mouse in a serum-free medium.
Article in Cell and tissue research, 1983. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 33 citations in OpenAlex.
- Development of a vitamin D-responsive organ culture system for adult and old rat intestine.Digestive diseases and sciences · 2002Article
- Analysis of structural and biochemical events occurring in the small intestine after dietary polyamine ingestion in suckling rats.Digestive diseases and sciences · 1996Article
- Positive influence of tetracycline on human fetal kidney in serum-free organ culture.In vitro cellular & developmental biology. Animal · 1994Article
- Fetal mouse kidney maturation in vitro: coordinated influences of epidermal growth factor, transferrin and hydrocortisone.Anatomy and embryology · 1991Article
- Cell proliferation in developing human stomach.Anatomy and embryology · 1990Article
- Organ culture of chicken cecum: morphologic and physiologic observations after 24 and 48 h of culture.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1987Article
- Human foetal kidney explants in serum-free organ culture.Anatomy and embryology · 1987Article
- Human fetal colon in organ culture.Anatomy and embryology · 1987Article
- Establishment of an animal model of ovalbumin sensitised mouse to study protein induced enteropathy.Gut · 1986Article
- Morphological studies of the goldfish hindgut mucosa in organ culture.Cell and tissue research · 1984Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proximal and distal parts of the small intestine of 8-day-old suckling mice can best be maintained for 48 h in a serum-free organ culture system, Leibovitz L-15, at room air and room temperature. As determined by light and electron microscopy, the villous architecture was preserved as well as the classical ultrastructure of the enterocytes. Incorporation of 3H-thymidine and 3H-leucine continued during the culture period, reflecting a sustained synthesis of DNA and proteins for at least 48 h. The hydrolytic activities of the brushborder membrane, namely of lactase (L), trehalase (T), glucoamylase (GA) and alkaline phosphatase (AlPase) were measured in the explants as well as the culture medium. The overall enzymatic activities were increased as compared to the controls. In the tissue, L, GA and T activities remained stable or even increased during culture while in the medium an accumulation of enzymatic activities was noted especially for GA an AlPase. These results show that the morphological as well as the functional integrity of the mucosa is preserved for at least 48 h when small intestine of suckling mice is cultured in a serum-free medium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.