ArticleThe Journal of cell biology1981
Immunoelectron microscope studies of membrane-microfilament interactions: distributions of alpha-actinin, tropomyosin, and vinculin in intestinal epithelial brush border and chicken gizzard smooth muscle cells.
Article in The Journal of cell biology, 1981. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 122 papers.
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Who cites it
122 citing papers in PubMed, 387 citations in OpenAlex.
- Induction of Smooth Muscle Differentiation in Fibroblasts by Modulation of Cytoplasmic Actin Ratio.International journal of molecular sciences · 2026Article
- Membrane adhesion junctions regulate airway smooth muscle phenotype and function.Physiological reviews · 2023Review
- Identification and Characterization of Novel circRNAs Involved in Muscle Growth of Blunt Snout Bream (International journal of molecular sciences · 2021Article
- Vinculin is critical for the robustness of the epithelial cell sheet paracellular barrier for ions.Life science alliance · 2019Article
- Arg kinase-binding protein 2 (ArgBP2) interaction with α-actinin and actin stress fibers inhibits cell migration.The Journal of biological chemistry · 2015Article
- Vinculin phosphorylation at Tyr1065 regulates vinculin conformation and tension development in airway smooth muscle tissues.The Journal of biological chemistry · 2014Article
- Effects of basic calponin on the flexural mechanics and stability of F-actin.Cytoskeleton (Hoboken, N.J.) · 2012Article
- Multiple-labelling immunoEM using different sizes of colloidal gold: alternative approaches to test for differential distribution and colocalization in subcellular structures.Histochemistry and cell biology · 2011Article
- Activation of vinculin induced by cholinergic stimulation regulates contraction of tracheal smooth muscle tissue.The Journal of biological chemistry · 2011Article
- Limited digestion of α-actinin in the presence of F-actin.Biophysics (Nagoya-shi, Japan) · 2011Article
- New insights into vinculin function and regulation.International review of cell and molecular biology · 2011Review
- Vinculin regulates cell-surface E-cadherin expression by binding to beta-catenin.Journal of cell science · 2010Article
- Cardiac-myocyte-specific excision of the vinculin gene disrupts cellular junctions, causing sudden death or dilated cardiomyopathy.Molecular and cellular biology · 2007Article
- Partial reversal of transformed fusiform phenotype by overexpression of calreticulin.Cellular & molecular biology letters · 2007Article
- Dynamic association between alpha-actinin and beta-integrin regulates contraction of canine tracheal smooth muscle.The Journal of physiology · 2006Article
- Actin-based motility of intracellular microbial pathogens.Microbiology and molecular biology reviews : MMBR · 2001Review
- Characterization of human palladin, a microfilament-associated protein.Molecular biology of the cell · 2001Article
- Isoforms of alpha-actinin from cardiac, smooth, and skeletal muscle form polar arrays of actin filaments.The Journal of cell biology · 2000Article
- Nectin/PRR: an immunoglobulin-like cell adhesion molecule recruited to cadherin-based adherens junctions through interaction with Afadin, a PDZ domain-containing protein.The Journal of cell biology · 1999Article
- Ponsin/SH3P12: an l-afadin- and vinculin-binding protein localized at cell-cell and cell-matrix adherens junctions.The Journal of cell biology · 1999Article
62 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The ultrastructural localization of three cytoskeletal proteins, alpha-actinin, tropomyosin, and vinculin, in the brush border of epithelial cells of chicken small intestine and the smooth muscle cells of chicken gizzard was studied by immunofluorescence and immunonelectron microscope labeling of frozen sections of lightly fixed, intact tissues. In the immunoelectron microscope studies, a recently described new type of electron-dense antibody conjugate, imposil-antibody, has been successfully used, along with ferritin-antibody conjugates, in single and double immunolabeling experiments. In the intestinal brush border shows that vinvulin is sharply confined to the junctional complex close to the membrane region of the zonula adherens, in distinct contrast to the more diffuse distributions of the other two proteins. In the smooth muscle cells, the labeling patterns show that vinculin is sharply confined to the membrane-associated dense plaques, closer to the membrane than the alpha-Actinin is also present in the cytoplastic dense bodies, from which vinculin is absent. Tropomyosin is present diffusely distributed in the cytoplasm, but absent from both dense plaques and dense bodies. These findings with the muscle cells demonstrate, therefore, that the dense plaques and dense bodies are chemically and structurally distinct entities. The results with both tissues, along with those in previous papers (Geiger, 1979, Cell. 18:193-205.; Geiger et al., 1980, Proc. Natl. Acad. Sci. U. S. A. 77:4127-4131), suggest that vinculin may play an important and widespread role in the linkage of actin-containing microfilament bundles to membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.