ArticleThe Journal of cell biology1984
Transcellular ion currents in the water mold Achlya. Amino acid proton symport as a mechanism of current entry.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 92 citations in OpenAlex.
- Electrical signaling in fungi: past and present challenges.FEMS microbiology reviews · 2025Review
- Harnessing Fungi Signaling in Living Composites.Global challenges (Hoboken, NJ) · 2024Article
- Electrochemical regulation of budding yeast polarity.PLoS biology · 2014Article
- Roles of calcium ions in hyphal tip growth.Microbiological reviews · 1993Review
- Calcium-induced conidiation in Penicillium cyclopium: calcium triggers cytosolic alkalinization at the hyphal tip.Journal of bacteriology · 1993Article
- Extension growth of the water mold Achlya: interplay of turgor and wall strength.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Hydrogen ion currents in rat alveolar epithelial cells.Biophysical journal · 1991Article
- To shape a cell: an inquiry into the causes of morphogenesis of microorganisms.Microbiological reviews · 1990Review
- Detection of extracellular calcium gradients with a calcium-specific vibrating electrode.The Journal of cell biology · 1990Article
- Article
- Ionic currents in morphogenesis.Experientia · 1988Review
- Modifications of extracellular electric and ionic gradients preceding the transition from tip growth to isodiametric expansion in the apical cell of the fern gametophyte.Plant physiology · 1988Article
- Transcellular proton current in Achlya bisexualis hyphae: relationship to polarized growth.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Membrane-bound Ca2+ distribution visualized by chlorotetracycline fluorescence during morphogenesis of soredia in a lichen.The Histochemical journal · 1988Article
- Transcellular ion currents and extension of Neurospora crassa hyphae.The Journal of membrane biology · 1988Article
- Electrophysiological properties of Achlya hyphae: ionic currents studied by intracellular potential recording.The Journal of cell biology · 1986Article
- Article
- Preliminary evidence for a cholinergic-like system in lichen morphogenesis.The Histochemical journalArticle
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Achlya, like other tip-growing organisms, generates an endogenous electrical current such that positive charge flows into the hyphal apex and exits from the trunk. The present study is concerned with the mechanism of current generation by hyphae growing in a defined, complete medium. The intensity of the current, measured in the extracellular medium with a vibrating probe, was unaffected by the removal of all the inorganic constituents of the growth medium. However, an increase in the external pH or the deletion of amino acids abolished the current. Removal of methionine alone diminished the current by two thirds. Hyphae also generated a longitudinal pH gradient in the extracellular medium; the region surrounding the tip was more alkaline than the bulk medium, whereas the region around the trunk was relatively acidic. These findings suggest that a flux of protons, dependent upon amino acids in the medium, carries current into the tip and creates the surrounding alkaline zone. The proton current appears to result from the transport of amino acids rather than their metabolism. Conditions that abolished the current also inhibited methionine uptake but had little effect on the respiratory rate. The findings imply a connection between the proton current and chemiosmotic energy transduction. We propose that protons flow into the hyphal tip through amino acid/proton symporters that are preferentially localized there. The proton flux energizes the uptake of amino acids into the growing zone and may also contribute to the polarization of hyphal growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.