ArticleThe Journal of cell biology1984
Interconversion of metaphase and interphase microtubule arrays, as studied by the injection of centrosomes and nuclei into Xenopus eggs.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
60 citing papers in PubMed, 221 citations in OpenAlex.
- Xenopus laevis Egg Extracts.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A genetic basis for facultative parthenogenesis in Drosophila.Current biology : CB · 2023Article
- Parthenogenesis in dipterans: a genetic perspective.Proceedings. Biological sciences · 2023Review
- Centrosomal and Non-centrosomal Functions Emerged through Eliminating Centrosomes.Cell structure and function · 2020Review
- Review
- SMARCA4-inactivating mutations increase sensitivity to Aurora kinase A inhibitor VX-680 in non-small cell lung cancers.Nature communications · 2017Article
- A Motor-Gradient and Clustering Model of the Centripetal Motility of MTOCs in Meiosis I of Mouse Oocytes.PLoS computational biology · 2016Article
- One to only two: a short history of the centrosome and its duplication.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Article
- Golgi-derived CLASP-dependent microtubules control Golgi organization and polarized trafficking in motile cells.Nature cell biology · 2009Article
- Spindle assembly in the absence of a RanGTP gradient requires localized CPC activity.Current biology : CB · 2009Article
- Spindle assembly in the oocytes of mouse and Drosophila--similar solutions to a problem.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2007Review
- A perfect funeral with no corpse.The Journal of cell biology · 2003Article
- Biparental inheritance of gamma-tubulin during human fertilization: molecular reconstitution of functional zygotic centrosomes in inseminated human oocytes and in cell-free extracts nucleated by human sperm.Molecular biology of the cell · 1999Article
- The yeast motor protein, Kar3p, is essential for meiosis I.The Journal of cell biology · 1997Article
- Real time observation of anaphase in vitro.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Live analysis of free centrosomes in normal and aphidicolin-treated Drosophila embryos.The Journal of cell biology · 1996Article
- Chromosomes initiate spindle assembly upon experimental dissolution of the nuclear envelope in grasshopper spermatocytes.The Journal of cell biology · 1995Article
- The impact of chromosomes and centrosomes on spindle assembly as observed in living cells.The Journal of cell biology · 1995Article
- Segregation of holocentric chromosomes at meiosis in the nematode, Caenorhabditis elegans.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1993Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have designed experiments that distinguish centrosomal , nuclear, and cytoplasmic contributions to the assembly of the mitotic spindle. Mammalian centrosomes acting as microtubule-organizing centers were assayed by injection into Xenopus eggs either in a metaphase or an interphase state. Injection of partially purified centrosomes into interphase eggs induced the formation of extensive asters. Although centrosomes injected into unactivated eggs (metaphase) did not form asters, inhibition of centrosomes is not irreversible in metaphase cytoplasm: subsequent activation caused aster formation. When cytoskeletons containing nuclei and centrosomes were injected into the metaphase cytoplasm, they produced spindle-like structures with clearly defined poles. Electron microscopy revealed centrioles with nucleated microtubules. However, injection of nuclei prepared from karyoplasts that were devoid of centrosomes produced anastral microtubule arrays around condensing chromatin. Co-injection of karyoplast nuclei with centrosomes reconstituted the formation of spindle-like structures with well-defined poles. We conclude from these experiments that in mitosis, the centrosome acts as a microtubule-organizing center only in the proximity of the nucleus or chromatin, whereas in interphase it functions independently. The general implications of these results for the interconversion of metaphase and interphase microtubule arrays in all cells are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.