ArticleThe Journal of cell biology1984
The brush border cytoskeleton is not static: in vivo turnover of proteins.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Evidence for changes in beta- and gamma-actin proportions during inner ear hair cell life.Cytoskeleton (Hoboken, N.J.) · 2015Article
- A two-photon FRAP analysis of the cytoskeleton dynamics in the microvilli of intestinal cells.Biophysical journal · 2005Article
- Article
- Calpain regulates enterocyte brush border actin assembly and pathogenic Escherichia coli-mediated effacement.The Journal of biological chemistry · 2003Article
- Dynamic assembly of surface structures in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- MYO1A (brush border myosin I) dynamics in the brush border of LLC-PK1-CL4 cells.Biophysical journal · 2002Article
- Dynamic effects of acid on Barrett's esophagus. An ex vivo proliferation and differentiation model.The Journal of clinical investigation · 1996Article
- Activation of protein kinase C isozymes is associated with post-mitotic events in intestinal epithelial cells in situ.The Journal of cell biology · 1994Article
- Golgi-derived vesicles from developing epithelial cells bind actin filaments and possess myosin-I as a cytoplasmically oriented peripheral membrane protein.The Journal of cell biology · 1993Article
- Changes in keratin expression during fetal and postnatal development of intestinal epithelial cells.The Biochemical journal · 1992Article
- Calmodulin dissociation regulates brush border myosin I (110-kD-calmodulin) mechanochemical activity in vitro.The Journal of cell biology · 1990Article
- Intestinal brush border revisited.Gut · 1989Review
- Tropomyosin distinguishes between the two actin-binding sites of villin and affects actin-binding properties of other brush border proteins.The Journal of cell biology · 1987Article
- The role of actin bundling proteins in the assembly of filopodia in epithelial cells.Cell adhesion & migrationReview
- A membrane reservoir at the cell surface: unfolding the plasma membrane to fuel cell shape change.BioarchitectureReview
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The shape and stability of intestinal epithelial cell microvilli are maintained by a cytoskeletal core composed of a bundle of actin filaments with several associated proteins. The core filaments are intimately associated with the overlying plasma membrane, in which there occur rapid turnover of proteins and constant incorporation of new membrane. Previous work has shown that starvation or inhibition of protein synthesis results in modulation of microvillar length, which indicates that there may be cytoskeletal protein turnover. We demonstrate herein, by means of in vivo pulse labeling with radioactive amino acids, that turnover of brush border cytoskeletal proteins occurs in mature absorptive cells. Turnover of cytoskeletal proteins appears to be quite slow relative to membrane protein turnover, which suggests that the turnover of these two microvillar compartments is not coupled. We thus conclude that cytoskeletal protein turnover may be a factor used to maintain normal length and stability of microvilli and that the cytoskeleton cannot be considered a static structure.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.