ArticleCell and tissue research1983
The cytoskeleton of microvilli of leech photoreceptors. A stable bundle of actin microfilaments.
Article in Cell and tissue research, 1983. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 17 citations in OpenAlex.
- Stereocilia Rootlets: Actin-Based Structures That Are Essential for Structural Stability of the Hair Bundle.International journal of molecular sciences · 2020Review
- Anti-actin immunoreactivity is retained in rhabdoms of Drosophila ninaC photoreceptors.Cell and tissue research · 1990Article
- Membrane-associated actin in the rhabdomeral microvilli of crayfish photoreceptors.The Journal of cell biology · 1984Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The microvilli of leech photoreceptors have diameters in the range of 60-100 nm. Each contains a bundle of microfilaments extending into the photoreceptor soma as a rootlet (Walz 1979 b). Apparent thicknesses of individual filaments are estimated to be 4-5 nm, consonant with those of identified actin filaments in the basement membranes of blowflies (Blest and De Couet 1983). Frozen sections of leech photoreceptors labelled with antibodies against scallop actin exhibited strong binding to the rootlet region but not to the microvilli, which are destroyed by the severe saponin or acetone extraction needed to permeabilise the preparation. NBD-phallacidin binds strongly but non-specifically to the photoreceptors and does not allow positive identification of F-actin. The cytoskeletons of the microvilli and rootlets are adequately preserved by conventional routines of fixation, and similar results were obtained when retinae were pretreated with either 0.5 mM Ca2+, 10 mM EGTA, 2 mM Ep-475 (a specific inhibitor of thiol proteases) or 2 mM Ep-475 combined with 0.5 mM Ca2+. Unlike the axial cytoskeletons of arthropod photoreceptor microvilli, those of the leech are stable to cellular insult.
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Registered trials
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