ArticleThe Journal of cell biology1983
Microinjection of fluorescent tubulin into dividing sea urchin cells.
Article in The Journal of cell biology, 1983. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 53 citations in OpenAlex.
- Measuring Mitotic Spindle and Microtubule Dynamics in Marine Embryos and Non-model Organisms.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A possible role of intracellular isoelectric focusing in the evolution of eukaryotic cells and multicellular organisms.Journal of molecular evolution · 2009Article
- A functional GFP fusion for imaging clathrin-mediated endocytosis.Traffic (Copenhagen, Denmark) · 2008Article
- Microtubules and microscopes: how the development of light microscopic imaging technologies has contributed to discoveries about microtubule dynamics in living cells.Molecular biology of the cell · 1998Review
- Midzone microtubule bundles are continuously required for cytokinesis in cultured epithelial cells.The Journal of cell biology · 1996Article
- Keratin incorporation into intermediate filament networks is a rapid process.The Journal of cell biology · 1991Article
- Biotin-tubulin incorporates into kinetochore fiber microtubules during early but not late anaphase.The Journal of cell biology · 1989Article
- Fluorescent microtubules break up under illumination.The Journal of cell biology · 1988Article
- Specific inhibition of endogenous beta-tubulin synthesis in Xenopus oocytes by anti-messenger oligodeoxynucleotides.Nucleic acids research · 1988Article
- An investigation of microtubule organization and functions in living Drosophila embryos by injection of a fluorescently labeled antibody against tyrosinated alpha-tubulin.The Journal of cell biology · 1987Article
- Interzone microtubule behavior in late anaphase and telophase spindles.The Journal of cell biology · 1987Article
- Microtubule dynamics in vivo: a test of mechanisms of turnover.The Journal of cell biology · 1987Article
- Dynamics of microtubule depolymerization in monocytes.The Journal of cell biology · 1986Article
- Determination of the intracellular state of soluble macromolecules by gel filtration in vivo in the cytoplasm of amphibian oocytes.The Journal of cell biology · 1986Article
- Polymerization of tubulin in vivo: direct evidence for assembly onto microtubule ends and from centrosomes.The Journal of cell biology · 1985Article
- Distribution of fluorescently labeled tubulin injected into sand dollar eggs from fertilization through cleavage.The Journal of cell biology · 1985Article
- Spindle microtubule dynamics in sea urchin embryos: analysis using a fluorescein-labeled tubulin and measurements of fluorescence redistribution after laser photobleaching.The Journal of cell biology · 1984Article
- Tubulin dynamics in cultured mammalian cells.The Journal of cell biology · 1984Article
- Assembly properties of fluorescein-labeled tubulin in vitro before and after fluorescence bleaching.The Journal of cell biology · 1984Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To follow the dynamics of microtubule (MT) assembly and disassembly during mitosis in living cells, tubulin has been covalently modified with the fluorochrome 5-(4,6-dichlorotriazin-2-yl)aminofluorescein and microinjected into fertilized eggs of the sea urchin Lytechinus variegatus. The changing distribution of the fluorescent protein probe is visualized in a fluorescence microscope coupled to an image intensification video system. Cells that have been injected with fluorescent tubulin show fluorescent linear polymers that assemble very rapidly and radiate from the spindle poles, coincident with the position of the astral fibers. No fluorescent polymer is apparent in other areas of the cytoplasm. When fluorescent tubulin is injected near the completion of anaphase, little incorporation of fluorescent tubulin into polymer is apparent, suggesting that new polymerization does not occur past a critical point in anaphase. These results demonstrate that MT polymerization is very rapid in vivo and that the assembly is both temporally and spatially regulated within the injected cells. Furthermore, the microinjected tubulin is stable within the sea urchin cytoplasm for at least 1 h since it can be reutilized in successive daughter cell spindles. Control experiments indicate that the observed fluorescence is dependent on MT assembly. The fluorescence is greatly diminished upon treatment of the cells with cold or colchicine agents known to cause the depolymerization of assembled MT. In addition, cells injected with fluorescent bovine serum albumin or assembly-incompetent fluorescent tubulin do not exhibit fluorescence localized in the spindle but rather appear diffusely fluorescent throughout the cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.