ArticleThe Journal of cell biology1983
Subcellular distribution of rhodamine-actin microinjected into living fibroblastic cells.
Article in The Journal of cell biology, 1983. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 53 citations in OpenAlex.
- Micron-scale supramolecular myosin arrays help mediate cytoskeletal assembly at mature adherens junctions.The Journal of cell biology · 2022Article
- A possible role of intracellular isoelectric focusing in the evolution of eukaryotic cells and multicellular organisms.Journal of molecular evolution · 2009Article
- Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella.The Journal of cell biology · 2008Article
- Tropomyosin inhibits ADF/cofilin-dependent actin filament dynamics.The Journal of cell biology · 2002Article
- Identification, purification, and characterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP (vasodilator-stimulated phosphoprotein).Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Thymosin beta 4 (Fx peptide) is a potent regulator of actin polymerization in living cells.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Mechanisms responsible for F-actin stabilization after lysis of polymorphonuclear leukocytes.The Journal of cell biology · 1992Article
- Chicken cardiac myofibrillogenesis studied with antibodies specific for titin and the muscle and nonmuscle isoforms of actin and tropomyosin.Cell and tissue research · 1991Article
- Chemoattractant-stimulated polymorphonuclear leukocytes contain two populations of actin filaments that differ in their spatial distributions and relative stabilities.The Journal of cell biology · 1990Article
- Mechanism of the formation of contractile ring in dividing cultured animal cells. I. Recruitment of preexisting actin filaments into the cleavage furrow.The Journal of cell biology · 1990Article
- Exogenous nucleation sites fail to induce detectable polymerization of actin in living cells.The Journal of cell biology · 1990Article
- Incorporation and turnover of biotin-labeled actin microinjected into fibroblastic cells: an immunoelectron microscopic study.The Journal of cell biology · 1989Article
- Assembly of different isoforms of actin and tropomyosin into the skeletal tropomyosin-enriched microfilaments during differentiation of muscle cells in vitro.The Journal of cell biology · 1986Article
- Role of stress fiber-like structures in assembling nascent myofibrils in myosheets recovering from exposure to ethyl methanesulfonate.The Journal of cell biology · 1986Article
- Probing the mechanism of incorporation of fluorescently labeled actin into stress fibers.The Journal of cell biology · 1986Article
- Behavior of a fluorescent analogue of calmodulin in living 3T3 cells.The Journal of cell biology · 1985Article
- Treadmilling, diffusional exchange and cytoplasmic structures.Journal of muscle research and cell motility · 1985Review
- Identical distribution of fluorescently labeled brain and muscle actins in living cardiac fibroblasts and myocytes.The Journal of cell biology · 1985Article
- The relationship between stress fiber-like structures and nascent myofibrils in cultured cardiac myocytes.The Journal of cell biology · 1984Article
- Microinjection of somatic cells with micropipettes: comparison with other transfer techniques.The Biochemical journal · 1984Review
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
The time course and pattern of incorporation of rhodamine-labeled actin microinjected into cultured fibroblastic cells were examined by fluorescence microscopy. Following microinjection, the fluorescent probe was incorporated rapidly into ruffling membranes, and within 5 min faintly fluorescent stress fibers were observed. Levels of fluorescence in ruffling membranes then tended to remain constant while fluorescence of the stress fibers continued to increase until approximately 20-min postinjection. Small, discrete regions of some microinjected cells displayed high levels of fluorescence that appeared initially approximately 5-10 min postinjection. I observed these small areas of intense fluorescence frequently near the cell periphery, which corresponded to focal contacts when examined with interference reflection optics. The results of this study show that a relationship exists between patterns of fluorescent actin incorporation in these cells and cellular areas or structures presumed to play a role in cell movement. These findings suggest that actin within stress fibers and the microfilament network of ruffling membranes undergoes a rapid turnover that may relate directly to the motility of the cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.